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ProteomicsProtein aggregation capture on microparticles enables multi-purpose proteomics sample preparation
Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-03-05 | PXD011677 | Pride
Microparticles Mass spectrometry Secretomics Proteomics Sample preparation Phosphoproteomics Magnetic beads 
ProteomicsRapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer
The faster sequencing speed available on the latest Q Exactive HF mass spectrometer is investigated and evaluated by four different acquisition methods and benchmarked across three generations of Q Exactive instruments. Also the instrument capabilities for offline high pH reversed-phase peptide frac...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-01 | MSV000079548 | MassIVE
OtherRapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer.
Data from ProteomeXchange, PXD ID: PXD001305. Experiment: UPLC9_RJC_DEV_HeLa_03, file: 20140418_QE7_UPLC9_RJC_DEV_HeLaFrac10_03.mzml. Published as part of J Proteome Res. 2014 Oct 28 . From the Abstract: {{i}} Here, we investigate faster sequencing speed of the latest Q Exactive HF mass spectromete...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM11210030961 | GPMDB
Resource Reanalysis 
OtherRapid and Deep Proteomes by Faster Sequencing on a Benchtop Quadrupole Ultra-High-Field Orbitrap Mass Spectrometer.
Data from ProteomeXchange, PXD ID: PXD001305. Experiment: UPLC9_RJC_DEV_HeLa_02, file: 20140418_QE7_UPLC9_RJC_DEV_HeLaFrac1_02.mzml. Published as part of J Proteome Res. 2014 Oct 28 . From the Abstract: {{i}} Here, we investigate faster sequencing speed of the latest Q Exactive HF mass spectrometer...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320004807 | GPMDB
Resource Reanalysis 
ProteomicsHeLa proteome of 12,250 protein-coding genes
We developed an optimized multi-shot proteomics workflow based on high-resolution offline high pH reversed-phase peptide separation of high peptide loads collecting many fractions that were in turn analyzed by short online chromatographic separations and fast peptide sequencing using orbitrap tandem...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000081563 | MassIVE
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