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RNA-seq of single mouse embryos using SMART-seq2. The embryos have been generated with IVF and are staged as hours post-IVF. At the zygote stage (~ 4h post-IVF), the embryos have been microinjected with 1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Non-injected embryos are denoted as...
ORGANISM(S): Mus musculus 
RNA-seq of single Fully Grown Oocytes using SMART-seq2. The oocytes were collected from female FVB mice, approximately. 44-48 h post-PMSG injection. The oocytes were microinjected with 1.3-1.7 μg/μL Kdm5b wild-type (WT) or catalytic mutant (CM) mRNA. Noninjected oocytes are denoted as “Noninj”. The ...
ORGANISM(S): Mus musculus 
Single-embryo SMART-seq of mouse Embryonic Genome Activation with 2-cell stage embryos collected after 2 h precision in vitro fertilization (IVF) at the following timepoints: 17, 20, 23, and 26 h post-IVF. Individual embryos were lysed in total cell lysis buffer, and the lysates were split to perfor...
ORGANISM(S): Mus musculus 
Insect derived cell-lines, from Spodoptera frugiperda (Sf21) and from Trichoplusia ni (High Five), are ones of the most widely used systems for recombinant protein expression in Baculoviral Expression Vector System (BEVS). Genomic sequences and annotations are still incomplete for Sf21 or absent for...
ORGANISM(S): Trichoplusia ni Spodoptera frugiperda 
2022-03-03 | PXD010282 | Pride
Correlation of miRNA expression patterns with clinically relevant information is greatly facilitated by the retrospective analysis of samples archived in tissue banks. Unfortunately, the quality of samples stored in tissue banks is variable due to heterogeneity in pre-analytical preparation of clini...
ORGANISM(S): Mus musculus 
Correlation of miRNA expression patterns with clinically relevant information is greatly facilitated by the retrospective analysis of samples archived in tissue banks. Unfortunately, the quality of samples stored in tissue banks is variable due to heterogeneity in pre-analytical preparation of clini...
ORGANISM(S): Mus musculus 
Abnormalities in metabolism of energetic substrates may play a role in progression of chronic heart failure (HF). The goal of the study was to examine the extent and mechanisms of metabolic alterations in rat model of chronic HF due to volume overload. Volume overload was induced in 3 months old mal...
ORGANISM(S): Rattus norvegicus 
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