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MetabolomicsMonitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry

RATIONALE: There is increasing interest in methods of direct analysis mass spectrometry that bypass complex sample preparation steps.

METHODS: One of the most interesting new ionisation methods is rapid evaporative ionisation mass spectrometry (REIMS) in wh...

2022-01-28 | MTBLS957 | MetaboLights
ProteomicsAmino acid and heavy water labelling to obtain protein turnover rates in intact adult animals
Both labelling studies were conducted with C57/BL6J adult male mice, derived from the same supplier and maintained under identical conditions. Eleven adult male C57BL/6JOlaHsd mice (Harlan UK Ltd., Shardlow, UK) of over 12 months of age at the start of the experiment were housed individually in 48 x...
ORGANISM(S): Mus Musculus (mouse) 
2021-12-13 | PXD029639 | JPOST Repository
ProteomicsQuantitative proteomics shows extensive remodelling induced by N limitation in Prochlorococcus sp. SS120 C4PR_LIV
Prochlorococcus is the most abundant photosynthetic organism on Earth, contributing significantly to the global primary production and playing a prominent role in biogeochemical cycles. Prochlorococcus requires the capability to accommodate to environmental changes in order to proliferate in oligotr...
ORGANISM(S): Prochlorococcus marinus subsp. marinus str. CCMP1375 
2017-05-03 | PXD005745 | Pride
Prochlorococcus N metabolism C metabolism 
ProteomicsCross-species proteomics in analysis of mammalian sperm proteins C4PR_LIV
Many proteomics studies are conducted in ‘model’ organisms for which a fully annotated and detailed, high quality proteome is available. By contrast, many studies in ecology and evolution lack a high quality proteome data set that can be used for protein discovery and quantification. This is particu...
ORGANISM(S): Bos taurus (Bovine) Mus musculus (Mouse) Sus scrofa domesticus (domestic pig) Rattus norvegicus (Rat) Equus caballus (Horse) 
2020-09-07 | PXD003164 | Pride
Rodents Sperm Lc-ms Cross-species Ungulates Proteins 
ProteomicsProtein turnover measurement using selected reaction monitoring-mass spectrometry (SRM-MS) C4PR_LIV
Protein turnover represents an important mechanism in the functioning of cells, with deregulated synthesis and degradation of proteins implicated in many diseased states. Therefore, proteomics strategies to measure, with high confidence, turnover rates are of vital importance to understanding many ...
ORGANISM(S): Mus musculus (Mouse) 
2016-08-10 | PXD004413 | Pride
Srm Proteomics Ms1 Mass spectrometry Protein turnover Quantification 
ProteomicsCharacterisation of urinary WFDC12 in nocturnal basal primates, mouse lemurs (Microcebus spp.), C4PR_LIV
Mouse lemurs are basal primates that rely on chemo- and acoustic signalling for social interactions in their dispersed social systems. We examined the urinary protein content of two mouse lemurs species, within and outside the breeding season, to assess candidates used in species discrimination, re...
ORGANISM(S): Microcebus murinus 
2017-02-13 | PXD005196 | Pride
Urine Whey acidic protein Mass spectrometry Wfdc12 protein Microcebus lehilahytsara Microcebus murinus Reproduction 
MetabolomicsRapid identification of species, sex and maturity by mass spectrometric analysis of animal faeces

BACKGROUND: We describe a new approach to the recovery of information from faecal samples, based on the analysis of the molecular signature generated by rapid evaporative ionisation mass spectrometry (REIMS).

RESULTS: Faecal pellets from five different r...

2022-02-03 | MTBLS1095 | MetaboLights
ProteomicsSynthetic biology meets proteomics: Construction of à la carte QconCATs for absolute protein quantification C4PR_LIV
We report a new approach to the assembly and construction of QconCATs, quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. The new approach is based on synthetic biology precepts of biobricks, making use of ...
ORGANISM(S): Escherichia coli 
2021-11-15 | PXD027521 | Pride
Synthetic biology Qconcat Quantitative proteomics Loop assembly 
ProteomicsStrategies to enhance the extraction of electrophoretically separated proteins from polyacrylamide gels and their application to mass spectrometry analyses (C4PR_LIV)
Polyacrylamide gel electrophoresis (PAGE) is a powerful technique for separating proteins extracted from complex biological samples. Unfortunately, the difficulty of recovering intact proteins in high yield from polyacrylamide matrices often limits further analyses. We discovered that staining prote...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Drosophila melanogaster (Fruit fly) 
2020-07-13 | PXD010592 | Pride
Protein extraction Sds page 
ProteomicsScentmarking communication in the female rat_C4PR_LIV
Chemical communication in mammals is in part mediated through the deposition of scent marks in the environment. Volatile and non-volatile moleculesin the scent marksconvey important information about the donor animal, such as sex, social and reproductive status,and stimulatea response in the recepto...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-05-29 | PXD039325 | Pride
Scents Mups Norway rat 
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