Finely ground leaf tissue of wild-type columbia-0 ecotype was used.
Protein extraction was performed in buffer-saturated phenol and high sucrose buffer.
Extracted proteins were incubated with purified recombinant GST or GST-BIN2 kinase.
Filter-assisted sample preparation (FASP) columns in prese...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)