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ProteomicsAn improved TurboID-based proximity labeling pipeline in C. elegans by biochemical depletion of endogenously biotinylated carboxylases
To review an improved proximity-labeling strategy that uses the improved E. coli biotin ligase TurboID to characterize C. elegans protein complexes, the interactome of a presynaptic active zone protein, ELKS-1, was analyzed as proof of principle. A significant constraint on the sensitivity of TurboI...
ORGANISM(S): Caenorhabditis elegans 
2022-09-12 | PXD034639 | Pride
Synapse Imac Apex2 Proximity-dependent protein labeling Hrp Biotinylated carboxylase depletion Turboid Caenorhabditis elegans 
UnknownDepletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans.
Not available
S-EPMC9437848 | biostudies-literature
UnknownControl of biotin biosynthesis in mycobacteria by a pyruvate carboxylase dependent metabolic signal.
Not available
S-EPMC5916780 | biostudies-literature
UnknownPharmacological induction of acetyl-CoA carboxylase 1 autophagic degradation attenuates lipid accumulation and cholangiocarcinoma progression.
Not available
S-EPMC12645744 | biostudies-literature
UnknownHost biotin is required for liver stage development in malaria parasites.
Not available
S-EPMC5856565 | biostudies-literature
TranscriptomicsExpression data by ACLY knockdown
De novo lipogenesis is activated in most cancers. Several lipogenic enzymes are implicated in oncogenesis and represent potential cancer therapeutic targets. RNA interference-mediated depletion of ATP citrate lyase (ACLY), the enzyme that catalyzes the first step of de novo lipogenesis, leads to gro...
ORGANISM(S): Homo sapiens 
2014-04-13 | E-GEOD-37243 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of butanol treated yeast to monitor the transcript level and also the translation status for each RNA
Global restriction of protein synthesis is a hallmark of cellular stress. Using fusel alchohol addition, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to seperate h...
ORGANISM(S): Saccharomyces cerevisiae 
2005-10-01 | E-MEXP-324 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of amino-acid starved yeast cells to monitor the transcript level and also the translation status for each RNA
Global restriction of protein synthesis is a hallmark of cellular stress. Using amino-acid starvation, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to seperate hig...
ORGANISM(S): Saccharomyces cerevisiae 
2005-10-01 | E-MEXP-323 | biostudies-arrayexpress
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