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ProteomicssBioSITe enables sensitive identification of the cell surface proteome through direct enrichment of biotinylated peptides
Background: Cell surface proteins perform critical functions related to immune response, signal transduction, cell-cell interactions, and cell migration. Expression of specific cell surface proteins can determine cell-type identity, and can be altered in diseases including infections, cancer and gen...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD044519 | Pride
Biosite Label-free quantitation Biotinylation Surfaceome 
ProteomicsAn improved TurboID-based proximity labeling pipeline in C. elegans by biochemical depletion of endogenously biotinylated carboxylases
To review an improved proximity-labeling strategy that uses the improved E. coli biotin ligase TurboID to characterize C. elegans protein complexes, the interactome of a presynaptic active zone protein, ELKS-1, was analyzed as proof of principle. A significant constraint on the sensitivity of TurboI...
ORGANISM(S): Caenorhabditis elegans 
2022-09-12 | PXD034639 | Pride
Synapse Imac Apex2 Proximity-dependent protein labeling Hrp Biotinylated carboxylase depletion Turboid Caenorhabditis elegans 
UnknownA simple elution strategy for biotinylated proteins bound to streptavidin conjugated beads using excess biotin and heat.
Not available
S-EPMC5693302 | biostudies-literature
ProteomicsUncover proteins binding HBV rcDNA
To comprehensively uncover host proteins involved in HBV rcDNA repair, we employed a rcDNA repair system, which can simulate the process of cccDNA formation in vitro. Biotinylated rcDNA first bonds with streptavidin magnetic beads and is then incubated with cell extracts of HepG2-NTCP-K7 cells to fa...
ORGANISM(S): Homo sapiens (Human) 
2024-12-18 | PXD053940 | Pride
Hbv rcdna Dna repair Hepatocytes 
TranscriptomicsTranscription profiling of rat colon exposed to soy or whey proteins
We previously reported that lifetime consumption of soy proteins or whey proteins reduced the incidence of azoxymethane (AOM)-induced colon tumors in rats. To obtain insights into these effects, global gene expression profiles of colons from rats with lifetime ingestion of casein (CAS, control diet)...
ORGANISM(S): Rattus norvegicus 
2009-04-30 | E-GEOD-6102 | biostudies-arrayexpress
GenomicsSequencing of DNA from the biotinylated chromatin from HeLa S3 clonal cell line
Sequencing of DNA from the biotinylated chromatin from HeLa S3 clonal cell line
PRJNA487642 | ENA
UnknownSignature Fragment Ions of Biotinylated Peptides.
Not available
S-EPMC7199424 | biostudies-literature
UnknownSimple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis.
Not available
S-EPMC2917173 | biostudies-literature
UnknownA Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins.
Not available
S-EPMC6474067 | biostudies-literature
UnknownUse of biotinylated plasmid DNA as a surrogate for HSV DNA to identify proteins that repress or activate viral gene expression.
Not available
S-EPMC3529035 | biostudies-literature
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