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ProteomicsBLM-G4 pulldown IP-MS in human colon cancer HT-29 cells
the experiment uses biotin-labelled BLM-G4 to incubate with HT-29 cell lysates, and uses streptavidin agarose beads to pulldown BLM-G4-binding proteins. Utilize LC-MS/MS to annotate and quantify the proteins pulled down by BLM-G4.
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075345 | Pride
Pulldown Blm-g4 Ip-ms Ht-29 cell 
TranscriptomicsaCGH analyses of DSB-induced LOH in Blm-deficient mESCs
Genome modification in mouse embryonic stem cells (mESCs) has provided the versatile platform to analyzing gene functions in mammalian cells. Bloom helicase-deficient mESCs showed elevated rate of loss of heterozygosity (LOH) through mitotic recombination. By utilizing this characteristic of Blm de...
ORGANISM(S): Mus musculus 
2012-11-30 | E-MTAB-1116 | biostudies-arrayexpress
ProteomicsEffect of TOPBEffect of TOPBP1 on BLM phosphosites
Identification of BLM phosphosites. The experiment is composed by 2 parts: i) identification of BLM phosphosites with DDA method ii) quantification of phosphosites with a targeted method.
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD026460 | Pride
Phosphosites 
ProteomicsIdentification BLM phosphosites in presence or absence of lambda phosphatase
Identification of BLM phosphosites in presence or absence of lambda phosphatase. DDA analysis of purified protein
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD026452 | Pride
Dda 
GenomicsChIP Sequencing of CRISPR/Cas9 corrected Bloom Syndrome patient cell line using antibody against BLM
BLM protein has been shown to play an important role in homologous recombination (HR) repair. We report that BLM interacts with RAD54 (another HR repair protein) to enhance its chromatin remodeling function. This experiment identifies that BLM gets recruited to different genetic loci even in the abs...
ORGANISM(S): Homo sapiens 
2024-04-03 | E-MTAB-11372 | biostudies-arrayexpress
ProteomicsBLM complex composition in unperturbed cells
The Bloom syndrome DNA helicase BLM contributes to chromosome stability through its roles in double-strand break repair by homologous recombination and DNA replication fork restart during the replication stress response. Loss of BLM activity leads to Bloom syndrome, which is characterized by extraor...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD018823 | Pride
Gm00637 Q exactive plus Human 
GenomicsA POLD3/BLM dependent pathway handles DSBs in transcribed chromatin upon excessive RNA:DNA hybrids accumulation
Transcriptionally active loci are particularly prone to breakage and mounting evidence suggest that DNA Double-Strand Breaks arising in active genes are handled by a dedicated repair pathway, Transcription-Coupled DSB Repair (TC-DSBR), that entails R-loop accumulation and dissolution. Here, we uncov...
ORGANISM(S): Homo sapiens 
2022-04-13 | E-MTAB-11592 | biostudies-arrayexpress
ProteomicsRole of BLM helicase in augmenting the phosphorylation of DNA damage response (DDR) proteins.
Assessing the importance of BLM helicase upon DNA damage in the phosphorylation enhancement of DNA damage response (DDR) proteins via phosphoproteomic profiling in the presence and absence of BLM.
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD048117 | Pride
Dna repair Atm Dna damage response Blm helicase 
TranscriptomicsDNA-damaging agent and immune inducer synergistically induced defence gene expression in Arabidopsis
We used microarrays to identify differentially expressed genes after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment. We focused on those genes that were synergistically induced by co-treatment (BLM+INA). Arabidopsis seedlings were treated with...
ORGANISM(S): Arabidopsis thaliana 
2013-11-14 | E-GEOD-44793 | biostudies-arrayexpress
ProteomicsThe Bloom’s syndrome helicase BLM functions as a homodimer within the Bloom’s complex
Homologous recombination (HR) is an essential and highly regulated cellular process. Unintended HR in somatic cells is potentially deleterious; it can result in translocations and/or somatic cell loss of heterozygosity. This is suppressed by the BLM protein in combination with topoisomerase III, RM...
ORGANISM(S): Homo sapiens (Human) 
2022-01-17 | PXD011078 | Pride
Blm Crosslinking ms Structural proteomics Bloom’s syndrome 
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