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ProteomicsQuantitative mass spectrometry analysis of intestinal organoids
Organoids have the potential to bridge 3D cell culture to tissue physiology by providing a model resembling in vivo organs. We propose here a SILAC method to measure protein expression changes in intestinal organoids under different experimental conditions. With the combined use of quantitative mass...
ORGANISM(S): Mus musculus (Mouse) 
2017-09-05 | PXD004607 | Pride
Intestine Organoids Silac Quantitative proteomics 
ProteomicsComprehensive characterization of Minichromosome Maintenance Complex (MCM) protein interactions using affinity and proximity purifications coupled to mass spectrometry.
This project is the complete interactome of the Minichromosome Maintenance (MCM) complex by using AP-MS and BioID approaches for all the different MCM proteins. Overall, our analysis identified unique and shared interaction partners and proteins enriched for distinct biological processes including D...
ORGANISM(S): Homo sapiens (Human) 
2016-08-09 | PXD004089 | Pride
Ap-ms Bioid Mcm complex 
ProteomicsInteractome of NudCD1 isoforms 1, 2 & 3
NudCD1, also known as CML66 or OVA66, is a 583 amino acids protein initially identified as overexpressed in patients with chronic myelogenous leukemia. The mRNA of NudCD1 is expressed in heart and testis of normal tissues, and is overexpressed in several cancers. Previous studies have shown that the...
ORGANISM(S): Homo sapiens (Human) 
2017-12-06 | PXD005493 | Pride
Human Cancer Nudcd1 Mrna splicing 
ProteomicsSubcellular proteomics analysis of different stages of colorectal cancer cell lines
Studying cell differentiation and transformation allows a better understanding of the mechanisms involved in the initiation and the evolution of cancer. The role of proteins which participate in these processes is dependent on their location within the cell. Determining the subcellular localization ...
ORGANISM(S): Homo sapiens (Human) 
2017-04-19 | PXD004879 | Pride
Subcellular proteomics Colorectal cancer Silac Colon 
ProteomicsAP-MS of GFP-MCM2 in the cellular response to etoposide induced DNA damage.
SILAC quantitative proteomics using immunoprecipitation of GFP-tagged fusion proteins to identify proteins interacting with the MCM complex, and quantify changes in interactions in response to DNA damage. U2OS cells expressing GFP-MCM2 were used to purify the MCM complex following treatment or not w...
ORGANISM(S): Homo sapiens (Human) 
2017-12-06 | PXD001949 | Pride
Dna repair Etoposide Mcm2 
ProteomicsDestabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks induced by Etoposide.
DNA replication during S phase involves thousands of replication forks that must be coordinated to ensure that every DNA section is only replicated once. The minichromosome maintenance proteins, MCM2 to MCM7, form a heteromeric DNA helicase required for both initiation of DNA replication and elongat...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD009539 | Pride
Dna repair Mcm protein Etoposide Dna damage Homologous recombination 
ProteomicsFunctions of the 12 different isoforms of the hepatocyte nuclear factor 4 alpha (HNF4α)
HNF4α is a nuclear receptor regulating the transcription of genes involved mainly in development, cell differentiation and metabolism. Opposite functions for the two classes of P1 and P2 isoforms of HNF4α have recently been highlighted. These classes include 12 variants of HNF4α that can be expresse...
ORGANISM(S): Homo sapiens (Human) 
2020-04-17 | PXD012146 | Pride
Transcription Proteomics Nuclear receptors Hnf4α Silac Isoforms Colorectal cancer 
ProteomicsThe response to neoadjuvant chemoradiotherapy to 5-fluorouracil in locally advanced colorectal cancer patients: a predictive proteomic signature.
Background: Colorectal cancer is the third most common and the fourth most lethal cancer in the world. In the majority of cases, patients are diagnosed at an advanced stage or even metastatic, thus explaining the high mortality. The standard protocol for treating patients with locally advanced non-m...
ORGANISM(S): Homo sapiens (Human) 
2018-06-25 | PXD008440 | Pride
5-fluorouracil Proteomics Colorectal cancer Resistance to neoadjuvant radio-chemotherapy Mass spectrometry Predictive biomarkers 
ProteomicsDownregulation of KRAB zinc finger proteins in 5-fluorouracil resistant colorectal cancer cells
The radio-chemotherapy with 5-fluorouracil (5-FU) is the standard of care treatment for patients with locally advanced rectal cancer (LARC) but it is only effective for a third of them. The proteomic and transcriptomic response of three colorectal cancer (CRC) cell lines to 5-FU and radiation was as...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD020000 | Pride
Colorectal cancer Tmt Protoemics 5-fu Chemoresistance 
ProteomicsIdentification of a highly sensitive combination of protein biomarkers for the detection of high-grade bladder cancer
Bladder cancer is the fifth most common malignancy worldwide and presents a significant diagnostic challenge due to its high recurrence rate and reliance on invasive detection methods such as cystoscopy. Current diagnostic tools, including cytology, have limited sensitivity. This study aims to ident...
ORGANISM(S): Homo sapiens (Human) 
2026-02-03 | PXD065950 | Pride
Proteomics Mass spectrometry Bladder cancer Non-invasive diagnostics Early detection Urinary biomarkers 
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