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GenomicsPost-licensing specification of eukaryotic replication origins by facilitated Mcm2-7 sliding along DNA
Eukaryotic genomes are replicated from many origin sites that are licensed by the loading of inactive double hexamers of the replicative DNA helicase, Mcm2-7. How eukaryotic origin positions are specified remains elusive. Here we show that, contrary to the bacterial paradigm, eukaryotic origins are ...
ORGANISM(S): Saccharomyces cerevisiae 
2016-01-07 | E-GEOD-69065 | biostudies-arrayexpress
TranscriptomicsChromatin IP for Mcm2-7, Rec8, Hop1 and Red1
Mcm2-7 ChIP in pre-meiotic and pre-mitotic cells, axis factor ChIP in wild-type and replication compromised strains in meiosis Multiple studies of meiotic chromosomes were undertaken. To study DNA replication, the locations of replicative helicase (Mcm2-7) were mapped in pre-meiotic and pre-mitotic ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35658 | biostudies-arrayexpress
TranscriptomicsKc167 Mcm2-7 Localization in HU
Determination of the genome-wide distribution of the Mcm2-7 helicase by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the beginning of S phase. Kc167 cells were arrested at the G1/S transition with hydroxyurea (HU). Goal was to evaluate changes in the genome-wide Mcm2-7 distri...
ORGANISM(S): Drosophila melanogaster 
2014-11-27 | E-GEOD-63680 | biostudies-arrayexpress
TranscriptomicsChIP-chip from Drosophila egg chambers using MCM2-7 antibody
Chromatin immunoprecipitation of the MCM2-7 replicative helicase followed by hybridization to genome-wide tiling microarrays demonstrated that MCMs localize to the follicle cell amplicons at stages of replication initiation Comparison of MCM2-7 ChIP sample compared to input DNA for Drosophila egg ch...
ORGANISM(S): Drosophila melanogaster 
2011-09-06 | E-GEOD-31889 | biostudies-arrayexpress
GenomicsChIP-Seq experiment of Mcm2-HA in wild type and H3K37R yeast cells
This ChIP-Seq experiment profiles Mcm2 binding to chromatin in wild type yeast cells and in cells with the K37R mutation in histone H3
ORGANISM(S): Saccharomyces cerevisiae S288C 
2021-04-28 | E-MTAB-9070 | biostudies-arrayexpress
ProteomicsAPEX2-MCM2 proximity labeling with high-resolution quantitative mass spectrometry in ESC
Through the APEX2 technique, we identified MCM2-interacting proteins associated with the replication initiation complex. After excluding proteins from the negative controls, we detected a substantial number of interacting proteins in the positive groups of both cell types. This research establishes ...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD054939 | Pride
Apex2  mcm2  dna replication  proximity labeling 
ProteomicsHistone dynamics at replication forks in embryonic stem cells
We employ inducible mutants to dissect the early and late consequences of impaired histone recycling. We find rapid accumulation of H3K27me3 in response to impaired histone recycling, preceding gene expression changes. Simultaneous disruption of both leading (POLE4) and lagging strand (MCM2-2A) recy...
ORGANISM(S): Mus musculus (Mouse) 
2025-01-12 | PXD053300 | Pride
Parental histones Pole4 Mcm2 Escs 
ProteomicsProteome of silenced minichromosome maintenance protein 2 with siRNA on non-small cell lung cancer H1299 cells
Lung cancer is the leading cause of cancer-related mortality. The two main lung cancer types are small cell lung cancer (SCLC) and non-SCLC (NSCLC), where NSCLC comprises about 80-85% of all lung cancer. Despite the introduction of improved treatments, the overall survival rate of lung cancer patien...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD003743 | Pride
Non small cell lung cancer h1299 cells Rna interference Proteome Minichromosome maintenance protein 2 Itraq Lc-ms/ms 
GenomicsProfiling of MCM2 and phosphorylated MCM2 in MLL3/4 double mutant and control ESCs [ChIP-seq]
Profiling of MCM2 and phosphorylated MCM2 in MLL3/4 double mutant and control ESCs [ChIP-seq]
PRJNA1003949 | ENA
TranscriptomicsChIP-Seq of yeast MCM2 in a time-course experiment after release from the G1 cell-cycle phase
ChIP-Seq experiment of MCM2 binding to chromatin in a time-course experiment after release from the G1 cell-cycle phase
ORGANISM(S): Saccharomyces cerevisiae S288C 
2021-04-28 | E-MTAB-9071 | biostudies-arrayexpress
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