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TranscriptomicsHuman BRAF-V600E melanoma cell lines drug sensitive vs drug resistant
aCGH of human melanoma cell lines comparing parental (drug sensitve) vs isogenic drug resistant-derived subline Two condition experiment: two BRAF-V600E mutant cell lines (drug sensitive - parental baseline) vs two derived sublines after chronic exposure to the MEK inhibitor trametinib (drug resista...
ORGANISM(S): Homo sapiens 
2014-04-21 | E-GEOD-49430 | biostudies-arrayexpress
TranscriptomicsRNA-seq melanoma
Using a chromatin regulator-focused shRNA library, we found that suppression of sex determining region Y-box 10 (SOX10) in melanoma causes resistance to BRAF and MEK inhibitors. To investigate how SOX10 loss leads to drug resistance, we performed transcriptome sequencing (RNAseq) of both parental A...
ORGANISM(S): Homo sapiens 
2014-01-04 | E-GEOD-50535 | biostudies-arrayexpress
GenomicsThe histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset
The most common mutation in human melanoma, BRAF(V600E), activates the serine/threonine kinase BRAF and causes excessive activity in the mitogen-activated protein kinase pathway. BRAF(V600E) mutations are also present in benign melanocytic naevi, highlighting the importance of additional genetic alt...
ORGANISM(S): Homo sapiens 
2011-03-25 | E-GEOD-28170 | biostudies-arrayexpress
ProteomicsRICTOR/mTORC2 downregulation in BRAF V600E melanoma cells promotes resistance to BRAF/MEK inhibition
Background Main drawback of BRAF/MEK inhibitors (BRAF/MEKi)-based targeted therapy in the management of BRAF- mutated cutaneous metastatic melanoma (MM) is the development of therapeutic resistance. We aimed to define in this context the specific role of mTORC2, a signaling complex defined by the pr...
ORGANISM(S): Homo sapiens (Human) 
2024-06-22 | PXD050614 | Pride
Targeted therapy Rictor Braf v600e melanoma Mitochondrial metabolism Drug resistance Nampt Mtorc2 
TranscriptomicsGene expression data from human metastatic melanoma tumors that may predict response to RAF265
To determine how mutation of BRAF affected the response to RAF265, we utilized a tumor orthotopic implant model of early passage melanoma tumors in nude mice from a series of 17 patients with advanced metastatic Tumor growth was compared between RAF265 treatment (40 mg/kg, QD) and diluent control gr...
ORGANISM(S): Homo sapiens 
2012-05-01 | E-GEOD-30812 | biostudies-arrayexpress
TranscriptomicsIdentification of direct transcriptional targets of V600EBRAF/MEK in melanoma
A375P melanoma cells were treated with 1uM of the MEK inhibitor PD184352 or 0.4uM of the V600EBRAF inhibitor PLX4720 for 2hr, 6hr and 24hrs. DMSO treatment for 2hr, 6hr and 24hrs serves as the negative control Triplicate experiments were performed for DMSO, PD184352 and PLX4720 treatment at 3 timepo...
ORGANISM(S): Homo sapiens 
2010-08-18 | E-GEOD-17089 | biostudies-arrayexpress
TranscriptomicsOncogenic BRAF regulates oxidative metabolism via PGC1alpha and MITF
We evaluated two matched human cell lines derived from primary melanocytes, termed pmel+BRAF(V600E) and pmel+BRAF(V600E)+MITF. These cells are therefore isogenic with the exception of the expression of MITF. Gene Set Enrichment Analysis of microarray data identified a highly significant induction of...
ORGANISM(S): Homo sapiens 
2013-03-07 | E-GEOD-38007 | biostudies-arrayexpress
TranscriptomicsExpression data from melanoma cell lines
Differential gene expression analysis of parental and resistant sub-lines of melanoma cell lines treated or untreated with PLX4032 Using microarray we sought to obtain a genome-wide profile of differentially expressed genes in parental melanoma cell lines and resistant sub-lines in response to PLX40...
ORGANISM(S): Homo sapiens 
2010-11-26 | E-GEOD-24862 | biostudies-arrayexpress
ProteomicsProteomic profiling by array of tyrosine kinase activity in metastatic malignant melanoma from human tissue specimens
Tyrosine kinase activity profiling of metatstatic malignant melanoma and normal skin tissue samples was performed using peptide kinase arrays. All samples were run in triplicates with and withouth inhbitor PLX4032 and Sutent Malate in order to identify how tyrosine kinases responds to kinase inhibit...
ORGANISM(S): Homo sapiens 
2013-01-17 | E-MTAB-1245 | biostudies-arrayexpress
TranscriptomicsOncogenic BRAFV600E remodels the melanocyte transcriptome and induces BANCR to regulate melanoma cell migration [HT-seq]
Most cancer genomics papers to date have focused on aberrations in genomic DNA and protein-coding transcripts. However, around 50% of transcripts have no coding potential and may exist as non-coding RNA. We performed RNA-seq in BRAFv600e melanoma skin cancer and on melanocytes over-expressing oncoge...
ORGANISM(S): Homo sapiens 
2012-04-28 | E-GEOD-33092 | biostudies-arrayexpress
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