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ProteomicsCross-linking mass spectrometry analyses of three different kinetochore protein complexes (KMN, NDC80C, MIS12C) using an MS-cleavable cross-linker, BuUrBu (DSBU)
In order to develop a simplified cross-linking mass spectrometry protocol, we applied one-step size-exclusion chromatography (SEC) for peptide purification after proteolysis with Lys-C and trypsin. Three benchmark protein complexes (KMN, NDC80C, MIS12C) from human kinetochore were cross-linked with ...
ORGANISM(S): Homo sapiens (Human) 
2019-04-01 | PXD010070 | Pride
Dsbu Q-exactive Cross-linking ms Buurbu Merox Human Kinetochore Protein complex Ms-cleavable cross-linker 
ProteomicsOGDH/DHTKD1-DLST (E1o/E1a- E2o) CL-MS
human OGDH (E1o) and DHTKD1 (E1a) were cross-linked with DLST (E2o). The samples were analyzed by LC-MS/MS in order to elucidate the interaction sites between these two E1-E2 subcomplexes.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2021-09-10 | PXD023525 | Pride
Dhtkd1 Ogdh 
ProteomicsCL-MS OF hE1a (DHTKD1) or G729R hE1a with hE2o
CL-MS OF hE1a (DHTKD1) or G729R hE1a with hE2o to identify the possible difference in the interactions between hE1a-hE2o and G729R hE1a-hE2o
ORGANISM(S): Homo sapiens (Human) 
2020-04-22 | PXD017792 | Pride
2-oxoadipate dehydrogenase Dhtkd1 
ProteomicsEpitope and Paratope Mapping of TNFα/Infliximab complex by cross-linking mass spectrometry and integrative modeling reveals high level of interaction specificity
Mass spectrometry (MS) has become one of the core technologies to study protein structures, protein-ligand and protein-protein interactions. Chemical cross-linking combined with mass spectrometry (XL-MS) is quickly spreading over different biochemistry laboratories for the continuous increase of its...
ORGANISM(S): Escherichia coli 
2024-02-27 | PXD043347 | Pride
Cross-linking mass spectrometry  monoclonal antibodies  integrative modeling  epitope/paratope mapping 
ProteomicsA cryo-EM structure of metazoan TRAPPIII, the multisubunit complex that activates the GTPase Rab1
The TRAPP complexes are nucleotide exchange factors that have essential roles in membrane traffic and autophagy. TRAPPII activates Rab11, and TRAPPIII activates Rab1, with the two complexes sharing a core of small subunits that affect nucleotide exchange, but being distinguished by specific large su...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-06-03 | PXD025064 | Pride
Exchange factor / rab1 / autophagy / membrane traffic / golgi 
ProteomicsPhospho-proteomic characterization of CCDC6-RET
Here we show that a CCDC6-RET fusion product, a driver and therapeutic target in lung and thyroid cancers, is a highly active dimeric kinase in solution. Time-resolved mass spectrometry analysis together with a robust biochemical and biophysical characterization reveal the autophosphorylation kineti...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD053907 | Pride
Ccdc6 Phosphorylation Ret 
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