Sort   by:  
 Page size 
GenomicsDNA accessibility profile of three C. elegans tissues during development
We show there is minimal genome-wide chromatin rearrangements (as measured by DNA accessibility) during tissue differentiation in C. elegans transgenic expression of E. coli DAM (DNA Adenine Methyltransferase) from tissue-specific promoters followed by mapping of methylated sites using a procedure t...
ORGANISM(S): Caenorhabditis elegans 
2011-04-16 | E-GEOD-23042 | biostudies-arrayexpress
GenomicsAmplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint
Exogenous double-stranded RNA (dsRNA) has been shown to exert homology-dependent effects at the level of both target mRNA stability and chromatin structure. Using C. elegans undergoing RNAi as an animal model, we have investigated the generality, scope, and longevity of chromatin-targeted dsRNA effe...
ORGANISM(S): Caenorhabditis elegans 
2012-01-06 | E-GEOD-32631 | biostudies-arrayexpress
TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
GenomicsChromatin organization in NPP-13 knockdown C. elegans embryos [seq]
C. elegans nuclear pore protein NPP-13 associates with small RNA genes transcribed by RNA Polymerase III. To test if the nuclear pore-chromatin interactions play a role in large-scale chromatin organization, we determined nuclear membrane-genome interactions and RNA Polymerase II localization in C. ...
ORGANISM(S): Caenorhabditis elegans 
2016-08-15 | E-GEOD-49903 | biostudies-arrayexpress
ProteomicsThe chromatin factors SET-26 and HCF-1 oppose the histone deacetylase HDA-1 in longevity and gene regulation in C. elegans
In this study, a GFP-tagged version of the protein HCF-1 was immuno-precipitated from C. elegans lysates of the strain IU171 and co-purifying proteins were identified by LC-MS/MS. As a negative control, the same immuno-precipitation was conducted from wild-type (strain N2) worms that did not carry a...
ORGANISM(S): Caenorhabditis elegans 
2024-01-29 | PXD047509 | Pride
Lc-msms C. elegans Immuno precipitation 
GenomicsEnrichment of H3K9me2 on unsynapsed chromatin in C. elegans does not target de novo sites [ChIP-Seq]
Characterization of the H3K9me2 distribution in the C. elegans adult hermaphrodite genome using ChIP-Seq Examination of H3K9me2 in two different nematode strains: fer-1 control and fer-1;him-8 mutant, where the X chromosomes are unsynapsed. The fer-1 mutation prevents production of embryos.
ORGANISM(S): Caenorhabditis elegans 
2015-09-04 | E-GEOD-67028 | biostudies-arrayexpress
TranscriptomicsFunction, targets and evolution of Caenorhabditis elegans piRNAs (small RNA-Seq)
piRNAs are required to maintain germline integrity and fertility but their mechanism of action is poorly understood. Here we demonstrate that C. elegans piRNAs silence transcripts in trans through imperfectly complementary sites. We find that target silencing is independent of Piwi endonuclease acti...
ORGANISM(S): Caenorhabditis elegans 
2012-06-18 | E-GEOD-28617 | biostudies-arrayexpress
GenomicsPartitioning the C. elegans genome by nucleosome modification, occupancy, and positioning
We have characterized two post-translational histone modifications in C. elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain pos...
ORGANISM(S): Caenorhabditis elegans 
2009-08-31 | E-GEOD-17284 | biostudies-arrayexpress
GenomicsHOT regions are CpG dense promoters in C. elegans and humans
ChIP seq of Cfp-1 and H3K4me3 in C. elegans late embryos The coding region of F52B11.1a (cfp-1) was PCR amplified from N2 genomic DNA using Phusion polymerase (Finnzymes) and Gateway cloned into pDONR221. The cfp-1 coding region was then recombined into the MosSCI compatible vector pCFJ201 (which ...
ORGANISM(S): Caenorhabditis elegans 
2014-01-01 | E-GEOD-49870 | biostudies-arrayexpress
TranscriptomicsChromatin organization in NPP-13 knockdown C. elegans embryos [array]
C. elegans nuclear pore protein NPP-13 associates with small RNA genes transcribed by RNA Polymerase III. To test if the nuclear pore-chromatin interactions play a role in large-scale chromatin organization, we determined nuclear membrane-genome interactions and RNA Polymerase II localization in C. ...
ORGANISM(S): Caenorhabditis elegans 
2016-08-15 | E-GEOD-49902 | biostudies-arrayexpress
Sort   by:  
 Page size