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ProteomicsPolylysine interactome in human cells
Polylysine chains are synthesized by ribosomes when a stop codon preceding the polyA tail on the mRNA fails to terminate the translation. To clarify the faith of the polylysine-tagged chains, we analyzed polylysine interactome in the cytosol of human 293T cells. We found an extensive network of inte...
ORGANISM(S): Homo sapiens (Human) 
2018-04-26 | PXD008171 | Pride
293t cells Polylysine Translation read-through 
ProteomicsFree mRNA interactome upon heat shock in Hela cells
The polypeptide-coding mRNAs are coated by specialized proteins at any stage of their lifecycle. One of few circumstances when free mRNA appears in the cytosol is the disassembly of polysomes during stress-induced shutdown of protein synthesis. Using quantitative mass spectrometry, we identified fre...
ORGANISM(S): Homo sapiens (Human) 
2019-06-28 | PXD012945 | Pride
Stress granules Mrna Proteostasis stress Rna granules 
ProteomicsInterference of RNA molecules with protein aggregation in vitro.
The analysis of RNA-protein interactomes indicates that the amount of structure in RNA molecules correlates with the capacity of binding to proteins in vitro and in vivo. To validate this observation, we used an in vitro network that mimics the composition of phase-separated RNA granules. The interf...
ORGANISM(S): Homo sapiens (Human) 
2019-09-13 | PXD011751 | Pride
B-isox Rna interactivity Rna granules 
ProteomicsInteractome of WT and mutant PUB1 in Saccharomyces cerevisiae
The yeast RNA binding protein PUB1 contains a prion-like domain (PrLD) and, when overexpressed, localizes into cytoplasmic liquid droplets and is toxic. However, truncated versions lacking the PrLD are either soluble or aggregating and do not significantly alter yeast growth. Analysis of the interac...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-02-04 | PXD011887 | Pride
Prion-like domain Rna granules Yeast Rbp 
ProteomicsSam68 interactome in mouse embryonic stem cells (mESCs)
Pellets from a mESC line with GFP tagged Sam68 and a control line (mESC expressing GFP alone) were collected at different time points during differentiation (Day 0, Day 3, Day 10).Pull-down was performed from normalized total cell lysates with GFP-trap beads (Chromotek) and analyzed by mass spectrom...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-08 | PXD009209 | Pride
Rna binding protein Stemm cells Lc-ms/ms 
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