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ProteomicsNovel insights into quantitative proteomics from an innovative bottom-up Simple Light Isotope Metabolic (bSLIM) labeling data processing strategy
The Simple Light Isotope Metabolic labeling (SLIM-labeling) is an inovative method to quantify proteome variations based on an original in vivo labeling strategy. Heterotrophic cells grown on a U-[12C] sole source of carbon synthesize U-[12C]-amino acids which are incorporated into proteins giving r...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-15 | PXD021329 | Pride
Openms Data processing workflow Yeast In vivo metabolic labeling Light carbon isotope Quantitative proteomics 12c Knime 
ProteomicsTraining set - Novel insights into quantitative proteomics from an innovative bottom-up Simple Light Isotope Metabolic (bSLIM) labeling data processing strategy
The Simple Light Isotope Metabolic labeling (SLIM-labeling) is an inovative method to quantify proteome variations based on an original in vivo labeling strategy. Heterotrophic cells grown on a U-[12C] sole source of carbon synthesize U-[12C]-amino acids which are incorporated into proteins giving r...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-02-16 | PXD021502 | Pride
Openms Data processing workflow Yeast In vivo metabolic labeling Light carbon isotope Quantitative proteomics 12c Knime 
TranscriptomicsTranscriptional effect of aft1 and aft2 mutation in iron depleted conditions
The paralogous transcription factors Aft1p and Aft2p activate the expression of genes involved in iron metabolism under iron depleted conditions. Both are able to bind to the same DNA consensus sequence in vitro. We used DNA microarrays and loss of function mutant strains to better understand the re...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-09 | E-GEOD-1763 | biostudies-arrayexpress
ProteomicsThe metacaspase Mca1p restricts O-glycosylation during farnesol-induced apoptosis in Candida albicans
Protein glycolysation is an essential posttranslational modification in eukaryotic cells. In pathogenic yeasts, it is involved in a large number of biological processes, such as protein folding quality control, cell viability and host/pathogen relationships. A link between protein glycosylation and ...
ORGANISM(S): Candida albicans (Yeast) 
2016-05-02 | PXD003677 | Pride
Candida albicans O-glycosylation Glycoproteomics Ssb1 N-glycosylation Metacaspase Bgl2 Farnesol Glycomics Cdc48 Aminoxytmt Erad Pdi1 Orbitrap fusion. Apoptosis Glycans quantification 
ProteomicsA proteomic and functional analysis of the metabolic adaptive response to iron during the filamentation process of the pathogen Candida albicans.
Candia albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to systemic diseases called candidiasis. Its ability to grow in various morphological forms, such as yeast and filamentous hyphae, contributes to its survival in the diverse microenvir...
ORGANISM(S): Candida albicans (Yeast) 
2020-05-26 | PXD010881 | Pride
Albicans-iron-filamentation 
ProteomicsImproved MS-based proteomics by the reduction of isotopic composition in vivo
Reducing the isotopic composition of proteins in vivo improves in-depth, high resolution MS-based quantitative proteomics. We used U-[12C]-glucose as the metabolic precursor of all amino acids in yeast. This substantially increased the peptide monoisotopic ion intensity in bottom-up analyses, greatl...
ORGANISM(S): Candida albicans (Yeast) 
2017-08-24 | PXD005364 | Pride
Protein half-life [u-12c]-carbon source Candida albicans Bottom-up ms-based proteomics 
ProteomicsNovel insights into redox-based mechanisms for auranofin- induced rapid cancer cell death
One of the significant features of cancer cells is a persistent pro-oxidative status. Therefore, compared to their normal counterparts, the malignant cells are generally more dependent on antioxidants for cell survival and more vulnerable to further oxidative insults via pharmacolog-ical interventio...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036558 | Pride
Auranofin  oxidative stress  redox regulation  redoxome  cancer 
ProteomicsThe adaptive metabolic response involves specific protein glutathionylation during the filamentation process in the pathogen Candida albicans
Candida albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to the systemic disease candidiasis. Its ability to adopt various morphological forms, such as unicellular yeasts, filamentous pseudohyphae and hyphae, contributes to its ability to s...
ORGANISM(S): Candida albicans (Yeast) 
2016-04-21 | PXD003685 | Pride
Candida albicans / filamentation/ glutathionylation/ isocitrate lyase / mitochondria 
ProteomicsIn vivo dynamics of Iron Starvation Induced Proteins under variable iron supplementation regimes suggest an iron processing organelle in Phaeodactylum tricornutum.
The model pennate diatom Phaeodactylum tricornutum is able to assimilate a range of iron sources. It therefore provides a platform to study different mechanisms of iron processing concomitantly in the same cell. In this study we follow the localisation of three iron starvation induced proteins (ISIP...
ORGANISM(S): Phaeodactylum tricornutum 
2023-03-10 | PXD021172 | Pride
Iron starvation induced proteins Fluorescent proteins Diatoms Siderophores Iron Proteome Phaeodactylum tricornutum 
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