Sort   by:  
 Page size 
ProteomicsPhotoaffinity capture compounds to profile the Magic Spot Nucleotide interactomes
Magic Spot Nucleotides (MSN) regulate the stringent response, a highly conserved bacterial stress adaptation mechanism, enabling survival when confronted with adverse external challenges. In times of antibiotic crisis, a detailed understanding of the stringent response is of critical importance, as ...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium Escherichia coli 
2026-07-27 | PXD032009 | Pride
Chemical proteomics Magic spot nucleotides (msn) Stringent response 
ProteomicsIdentifying SAM-binding proteins in D.melanogaster
The aim of the project is to identify proteins the ustilize S-adenosyl methionine as cofactor for performing methylation of target molecules in D. melanogaster S2 cells. For this purpose, we applied a small-molecule capturing protocol using a SAH-capture compound with UV-activated crosslinker and bi...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-03-11 | PXD005012 | Pride
Capture compound Fragmentation Methylation S-adenosyl methionine 
TranscriptomicsExpression data from LCM captured prostate cancer cells
The prostate represents a complex mix of cell types and there is a need to analyze distinct cell populations to better understand their potential interactions. This study of cell-type specific gene expression patterns will contribute to understanding of how tumor epithelial cells may be affected by...
ORGANISM(S): Homo sapiens 
2010-04-10 | E-GEOD-20758 | biostudies-arrayexpress
ProteomicsFishing for SAM/SAH-binding proteins
The aim of the project was to identify S-adenodsyl methionine-binding proteins expressed in D. melanogaster S2 cells. For this puprose a capture compund reagent carrying a SAH-affinity group, a biotin group for enrichment and a cross-linking moiety consisting of either benzophenone or a nitrobenzen...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-02-25 | PXD006245 | Pride
Capture compound S-adenosyl-methionine Lysine methylation Methyltransferase 
TranscriptomicsRNA-seq from tibialis muscles from rats aged 15 months (adult) and 27 months (old)
To gain new insights into molecular changes in skeletal muscle aging and disease with a special focus on differential alternative splicing and senescence, we performed RNA-seq on tibialis muscles from 15 months old (adult, a) and 27 months old (old, o) rats. Whole (w) muscle samples were prepared fo...
ORGANISM(S): Rattus norvegicus 
2021-06-26 | E-MTAB-10582 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of serum microRNAs in rat livers to screen for specific biomarkers to be used in diagnosis of compound- and herb-induced liver injury.
In this study, we employed a strategy to screen miRNA expression profiles in liver tissue by miRCURY LNA(tm) microRNA array analysis followed by TaqMan probe-based quantitative reverse transcription-PCR (qRT-PCR) to validate the miRNA expression profiles in serum and liver of two parallel rat drug-i...
ORGANISM(S): Rattus norvegicus 
2012-09-08 | E-MEXP-3364 | biostudies-arrayexpress
UnknownSmall molecule in situ resin capture provides a compound first approach to natural product discovery.
Not available
S-EPMC11187115 | biostudies-literature
UnknownSmall Molecule in situ Resin Capture - A Compound First Approach to Natural Product Discovery.
Not available
S-EPMC10312467 | biostudies-literature
TranscriptomicsSpatially distinct neutrophil responses within the inflammatory lesions of pneumonic plague
During pneumonic plague, the bacterium Yersinia pestis elicits the development of inflammatory lung lesions that continue to expand throughout infection. This lesion development and persistence is poorly understood. Here, we examine spatially distinct regions of lung lesions using laser capture micr...
ORGANISM(S): Mus musculus 
2015-07-13 | E-GEOD-70819 | biostudies-arrayexpress
ProteomicsInteraction-based capture and identification of protein effectors of ppGpp, the master regulator of bacterial growth
The small nucleotide ppGpp is a conserved regulatory molecule in prokaryotes that tunes growth rate in response to nutrient availability. How ppGpp regulates growth remains poorly defined. In Escherichia coli, ppGpp directly binds RNA polymerase to modulate transcription, but it likely has other tar...
ORGANISM(S): Escherichia coli 
2018-10-04 | PXD010402 | Pride
E. coli Ppgpp Lc-ms/ms 
Sort   by:  
 Page size