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TranscriptomicsSequencing of reaction products from protospacer integration by Cas1-Cas2
We report the sites of protospacer integration into plasmids by Cas1-Cas2. The goal of this study is to determine the role of sequence elements on protospacer integration. Methods: Cas1-Cas2 integration products were fragmented, end-repaired, adapter ligated, PCR amplified, and analyzed by Illumina ...
ORGANISM(S): Cloning vector pUC19 
2015-01-30 | E-GEOD-64552 | biostudies-arrayexpress
MetabolomicsCRISPR-Cas9 knockout of WS1A/WS1B alleviates postharvest green vein defect via tissue-specific chloroplast degradation in flue-cured tobacco
This dataset contains tissue-resolved untargeted metabolome data from flue-cured tobacco cultivar Honghuadajinyuan (wild-type) and transgene-free ws1 mutants generated by CRISPR-Cas9 knockout of homeologous WS1A/WS1B. Multiple tobacco tissues were profiled to capture metabolite alterations triggered...
2026-10-10 | MTBLS15979 | MetaboLights
ProteomicsCas1 and Fen1 Display Commutable Functions During Archaeal DNA Damage Repair
CRISPR-Cas constitutes an adaptive prokaryotic defence system against invasive nucleic acids like viruses and plasmids. Beyond their role in immunity, CRISPR-Cas systems have been shown to closely interact with components of cellular DNA repair pathways either by regulating their expression or via d...
ORGANISM(S): Haloferax volcanii DS2 
2022-05-20 | PXD029952 | Pride
Crispr Fen1 Archaea Dna damage Cas1 
ProteomicsCas1-Cas2 and DnaK physically and functionally interact to modulate CRISPR Adaptation
Prokaryotic Cas1-Cas2 protein complexes generate adaptive immunity to mobile genetic elements (MGEs), by capture and integration of MGE DNA in to CRISPR sites. De novo immunity relies on Cas1-Cas2 targeting MGE DNA, without the aid of pre-existing immunity complexes, through mechanisms of ‘naive ada...
ORGANISM(S): Escherichia coli 
2023-05-18 | PXD042090 | Pride
Crispr Cas1-cas2 Adaptaion Dnak 
GenomicsDraft genome sequence of Frankia sp. Cas1
Draft genome sequence of Frankia sp. Cas1
PRJEB65032 | ENA
OtherProtospacer integration into plasmid DNA by Cas1–Cas2
We report the integration site specificity of Cas1–Cas2 into target plasmid DNA encoding a CRISPR locus. The goal of this study is to determine the effect of IHF on target site selection of Cas1–Cas2.
ORGANISM(S): Cloning vector pUC19 
2016-05-27 | GSE79992 | GEO
OtherSequencing of reaction products from protospacer integration by Cas1-Cas2
We report the sites of protospacer integration into plasmids by Cas1-Cas2. The goal of this study is to determine the role of sequence elements on protospacer integration.
ORGANISM(S): Cloning vector pUC19 
2015-01-30 | GSE64552 | GEO
UnknownTargeted assemblies of cas1 suggest CRISPR-Cas's response to soil warming.
Not available
S-EPMC7305122 | biostudies-literature
GenomicsWhole-genome sequencing reveals recent transmission of mul-tidrug-resistant Mycobacterium tuberculosis CAS1-Kili strains in Lusaka, Zambia
An investigation of recent transmission of multidrug-resistant Mycobacterium tuberculosis CAS1-Kili strains in Lusaka, Zambia
PRJEB49247 | ENA
UnknownGlobal phylogenomic novelty of the Cas1 gene from hot spring microbial communities.
Not available
S-EPMC9755687 | biostudies-literature
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