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This study was carried out to identify the interacting partners of Leishmania donovani Cdc45, a protein that is conserved in eukaryotic DNA replication. Accordingly, Cdc45 was expressed in Leishmania promastigotes with a C terminal FLAG tag and whole cell lysates were isolated from these Ld1S transf...
ORGANISM(S): Leishmania donovani 
2025-10-27 | PXD064001 | Pride
The aim of the project was to purify Xenopus replisome from replicationg chromatin assembled in Xenopus laevis egg extract. To this end recombinant Cdc45-TEV-His10-FLAG5 was expressed in bacteria and purified. 4 ml of Xenopus laevis egg extract was activated into interphase and supplemented with 10 ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2023-10-27 | PXD044422 | Pride
Mechanisms of MCM2-7 loading and initial DNA melting at near base-pair resolution [ssDNA PIP-Seq]
Type of experiment: ChIP-chip (Chromatin immunoprecipitation on DNA chip) analyses of replication related, checkpoint related proteins and BrdU incorporated regions at 300bp resolution. Newly developed S.cerevisiae chromosome VI tiling array produced by affymetrix was used. Experimental factors:Dis...
ORGANISM(S): Saccharomyces cerevisiae 
Type of experiment: ChIP-chip (Chromatin immunoprecipitation on DNA chip) analyses of replication related, checkpoint related proteins and BrdU incorporated regions at 300bp resolution. Newly developed S.cerevisiae chromosome VI tiling array produced by affymetrix was used. Experimental factors:Dist...
ORGANISM(S): Saccharomyces cerevisiae 
2003-08-27 | GSE486 | GEO
Replication and Protein binding profile of chromosome VI of S.cerevisiae
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