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ProteomicsAnalysis of proteins immunoprecipitated with anti-FLAG antibodies from Leishmania donovani promastigotes expressing Cdc45-FLAG or carrying empty vector
This study was carried out to identify the interacting partners of Leishmania donovani Cdc45, a protein that is conserved in eukaryotic DNA replication. Accordingly, Cdc45 was expressed in Leishmania promastigotes with a C terminal FLAG tag and whole cell lysates were isolated from these Ld1S transf...
ORGANISM(S): Leishmania donovani 
2025-10-27 | PXD064001 | Pride
Lc-ms Leishmania donovani Immunoprecipitation Cdc45-flag 
ProteomicsXenopus laevis Cdc45-FLAG interactions on chromatin during DNA replication
The aim of the project was to purify Xenopus replisome from replicationg chromatin assembled in Xenopus laevis egg extract. To this end recombinant Cdc45-TEV-His10-FLAG5 was expressed in bacteria and purified. 4 ml of Xenopus laevis egg extract was activated into interphase and supplemented with 10 ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2023-10-27 | PXD044422 | Pride
Dna replication Xenopus laevis Cdc45 Replisome 
GenomicsChIP-on-chip of Cdc45 in S. pombe
ChIP-on-chip of Cdc45 in S. pombe
PRJNA427325 | ENA
UnknownSld3CBD-Cdc45 structural insights into Cdc45 recruitment for CMG complex formation during DNA replication.
Not available
S-EPMC12416888 | biostudies-literature
TranscriptomicsOrigin-Dependent DNA Replication in vitro: Insights into DDK and S-CDK function and Replisome Assembly
Initiation of eukaryotic DNA replication requires temporal separation of helicase loading from helicase activation and replisome assembly. Using an in vitro assay for eukaryotic origin-dependent replication initiation, we investigated the control of these events. After helicase loading, we found t...
ORGANISM(S): Saccharomyces cerevisiae 
2011-07-21 | E-GEOD-29646 | biostudies-arrayexpress
OtherChIP-on-chip of Cdc45 in S. pombe
ChIP-on-chip of Cdc45 in synchronized cells operating with the analog-sensitive CDK fusion protein (Cdc13-Cdc2). Cells were released from a G2 block or from a G2 block followed by an extended G1 (G1+ 15 min extension). For the G2 block, samples were collected 30 minutes after release. For the G1+15 ...
ORGANISM(S): Schizosaccharomyces pombe 
2018-02-04 | GSE108453 | GEO
UnknownHuman DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.
Not available
S-EPMC4439256 | biostudies-literature
UnknownInitiation-specific alleles of the Cdc45 helicase-activating protein.
Not available
S-EPMC6435160 | biostudies-literature
UnknownDNA replication protein Cdc45 directly interacts with PCNA via its PIP box in Leishmania donovani and the Cdc45 PIP box is essential for cell survival.
Not available
S-EPMC7255605 | biostudies-literature
UnknownHigh abundance of CDC45 inhibits cell proliferation through elevation of HSPA6.
Not available
S-EPMC9251052 | biostudies-literature
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