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MetabolomicsMetabolic Dynamics in Escherichia coli-Based Cell-Free Systems
The field of metabolic engineering has yielded remarkable accomplishments in using cells to produce valuable molecules, and cell-free expression (CFE) systems have the potential to push the field even further. However, CFE systems still face some outstanding challenges, including endogenous metaboli...
2023-02-22 | MTBLS2630 | MetaboLights
MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
MetabolomicsSerine synthesis pathway enzyme PHGDH is critical for muscle cell biomass, anabolic metabolism and mTORC1 signaling

Cells use glycolytic intermediates for anabolism e.g., via the serine synthesis and pentose phosphate pathways. However, we still understand poorly how these metabolic pathways contribute to skeletal muscle cell biomass generation. The first aim of this study was therefore to identify enzymes tha...

2025-03-21 | MTBLS8854 | MetaboLights
MetabolomicsPFKFB3 activates CAD to enhance de novo pyrimidine synthesis for cell growth

Aerobic glycolysis, termed the Warburg effect, is one of the aberrant metabolic pathways in highly proliferating cells. Glycolysis provides glycolytic metabolites to support the generation of biomass, such as nucleotides, amino acids, and lipids. To date, research on the direct interactions betwe...

2025-07-08 | MTBLS12688 | MetaboLights
UnknownReconstituted cell-free protein synthesis using in vitro transcribed tRNAs.
Not available
S-EPMC7334211 | biostudies-literature
TranscriptomicsAbsolute quantification of protein production reveals principles underlying protein synthesis rates
A quantitative view of cellular functions requires precise measures of the rates of biomolecule production, especially proteins-the direct effectors of biological processes. Here we present a genome-wide approach, based on ribosome profiling, for measuring absolute protein synthesis rates. The resul...
ORGANISM(S): Escherichia coli 
2014-04-24 | E-GEOD-53767 | biostudies-arrayexpress
ProteomicsEngineering cell-free systems by chemoproteomic-assisted phenotypic screening.
Phenotypic screening is a valuable tool to both understand and engineer complex biological systems. We demonstrate the functionality of this approach in the development of cell-free protein synthesis (CFPS) technology. Phenotypic screening identified numerous compounds that enhanced protein produc...
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2024-07-22 | PXD047986 | JPOST Repository
ProteomicsEscherichia coli-based cell-free reactions in different oxygen concentrations
Escherichia coli-based cell-free protein synthesis reactions conducted in precisely contolled oxygen concentrations within tube-in-tube reactor. The cell-free reactions after 4-hour incubation in 0%, 21%, and 100% oxygen were analysized by HPLC-MS/MS.
ORGANISM(S): Escherichia Coli 
2020-03-16 | PXD018058 |
ProteomicsIn vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity
The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the PURE system-catalyzed generation of RNAs and proteins constituting the PURE system itself represents a major challenge toward a self-replica...
ORGANISM(S): Escherichia Coli 
2021-09-06 | PXD020877 | panorama
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