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TranscriptomicsGenomewide measurements of whole embryo mRNA decay in Drosophila
whole embryo (all tissues) measurement of mRNA decay by 4-thiouridine pulse-chase These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Measurements with or without transcription inhibition by actinomycin D (ActD) were compared.
ORGANISM(S): Drosophila melanogaster 
2015-03-31 | E-GEOD-67435 | biostudies-arrayexpress
TranscriptomicsGenomewide measurements of neural-specific mRNA decay in Drosophila embryos
Neural-specific mRNA decay measurements by TU-Decay technique in control and Pumilio knockdown embryos These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Neural-specific RNA purification was achieved using prospero-GAL4 drivin...
ORGANISM(S): Drosophila melanogaster 
2015-04-02 | E-GEOD-67512 | biostudies-arrayexpress
TranscriptomicsThiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data
Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for...
ORGANISM(S): Toxoplasma gondii 
2005-07-16 | E-GEOD-2947 | biostudies-arrayexpress
TranscriptomicsThiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data
Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for pulse chase ...
ORGANISM(S): Toxoplasma gondii 
2005-07-16 | GSE2947 | GEO
GenomicsEC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons
EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons
PRJNA883697 | ENA
TranscriptomicsTranscription profiling of Toxoplasma gondii thiouracil pulse-chase, tachyzoites and bradyzoites mRNA stability data
Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for pulse cha...
ORGANISM(S): Toxoplasma gondii 
2005-07-07 | E-SMDB-2949 | biostudies-arrayexpress
ProteomicsA novel pulse-chase SILAC strategy measures changes in protein decay and synthesis rates induced by perturbation of proteostasis with an Hsp90 inhibitor.
We developed a pulse-chase variant of SILAC (stable isotope labeling by amino acids in cell culture pcSILAC). pcSILAC can quantitate in one experiment and for two conditions the relative levels of proteins newly synthesized in a given time as well as the relative levels of remaining preexisting prot...
ORGANISM(S): Homo sapiens (Human) 
2013-12-10 | PXD000538 | Pride
Hsp90 T-cells Pulse-chase Silac Synthesis rate Decay rate 
GenomicsSingle cell methylation of dormant and active hematopoietic stem cell and multipotent progenitor from H2B-GFP bone marrow after 100 days of chase
Single cell Methylome and Transcriptome Sequencing (scM&T-Seq) was performed on index-sorted single CD48- CD135- Lin- Sca-1+ c-Kit+ cells from Scl-tTA; H2B-GFP mouse bone marrow after 100 days of chase. Methylation data is uploaded here.
ORGANISM(S): Mus musculus 
2025-08-06 | E-MTAB-15477 | biostudies-arrayexpress
Proteomics3T3-L1 adipocyte protein turnover
Insulin is a potent regulator of protein metabolism. Here we describe a time-resolved map of insulin-regulated protein turnover in 3T3-L1 adipocytes using metabolic pulse-chase labelling and high-resolution mass spectrometry.
ORGANISM(S): Mus musculus (Mouse) 
2016-10-07 | PXD003696 | Pride
Proteostasis Pulse-chase silac Protein synthesis Insulin 
UnknownControlling Hypertension After Severe Cerebrovascular Event (CHASE): study protocol for a randomized controlled trial.
Not available
S-EPMC5822484 | biostudies-literature
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