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ProteomicsDifferent expressed proteins of HIV-infected long-term non-progressors (LTNPs) and regular progressors (RPs)
The whole blood samples were obtained from 3 LTNPs and 2 RPs HIV-1 infected patients, and the CD4+ T cells were isolated using anti-CD4-specific antibody-coated microbeads (130-045-101, Miltenyi Biotec, Germany) according to the manufacturer's instructions. Then the proteins extracted from the CD4+ ...
ORGANISM(S): Homo Sapiens 
2023-10-12 | PXD046078 |
ProteomicsHost proteins interacting with EV71-VP2 were identified by mass spectrometry
EV71-VP2-MYC was extransfected into 293T cells, and 36 hours later, the cells were treated with MG132 for 12 hours. The cells were harvested, and the cell lysates were incubated with protein-G with MYC antibody overnight. The interacting proteins were analyzed by mass spectrometry.
ORGANISM(S): Homo Sapiens 
2025-01-07 | PXD059489 |
ProteomicsIdentification of host factors interacting with HIV-1 Tat
The promiscuous biotin ligase TurboID was fused to Tat for precise and temporal biotinylation of surrounding proteins, which could be enriched by streptavidin and identified by mass spectrometry.
ORGANISM(S): Homo Sapiens 
2025-01-03 | PXD059409 |
ProteomicsDifferent expressed proteins of HIV-infected long-term non-progress (LTNPs) and regular progressors (RPs)
To identify potential host factors promoting HIV-1 control and non-progressive infection, we employed mass spectrometry-based proteomic profiling to analyze three LTNPs and two PRs，in order to identify proteins expressed at higher levels in LTNPs.
ORGANISM(S): Homo Sapiens 
2024-05-30 | PXD050294 |
ProteomicsProteins interacting with SARS-CoV-2 envelope
HEK293T were transfected with SARS-CoV-2-E-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for pr...
ORGANISM(S): Homo Sapiens 
2023-01-01 | PXD039140 |
TranscriptomicsSKBR3 cells treated with epirubicin or hypoxia
Intratumoral heterogeneity may generate a diversity of resistance mechanisms that could cause different therapeutic responses in different cell populations. Gene expression profiles were generated for breast cancer cells that had survived two different treatment and were also sorted by the cancer st...
ORGANISM(S): Homo sapiens 
2015-09-09 | E-GEOD-72362 | biostudies-arrayexpress
ProteomicsHuman trabecular meshwork LC-MS/MS
The trabecular meshwork (TM) responsible for IOP homeostasis in the eye could sense the IOP fluctuations dynamically and adapt to the mechanical changes appropriately. Cationic mechanosensitive channels (CMCs) have been reported with critical roles in mediating the TM responding to many mechanical f...
ORGANISM(S): Homo Sapiens 
2022-03-14 | PXD032288 |
ProteomicsComprehensive Analysis of the Glycome and Glycoproteome of Bovine Milk-Derived Exosomes
Bovine milk-derived exosomes (BMDEs) have potential applications in the pharmaceutical industry as drug delivery carriers. A comprehensive analysis of protein glycosylation in exosomes is necessary to elucidate the process of target delivery. In this work, free oligosaccharides (FOSs), O-glycans and...
ORGANISM(S): Bos Taurus 
2021-05-07 | PXD025847 |
ProteomicsSimultaneous profiling of phosphorylation and S-nitrosylation during maize seed germination
We developed a phosphate-affinity tag-switch (PAT-switch) proteomics workflow enabling simultaneous, site-specific profiling of phosphorylation and S-nitrosylation during maize seed germination.
ORGANISM(S): Zea Mays 
2026-01-21 | PXD073327 |
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