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HeLa cells were transfected with STK38 siRNA or Luc siRNA and then treated with EBSS or LLOMe in three biological replicates. The cells were lysed in 500 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (330...
ORGANISM(S): Homo Sapiens (human) 
Transcriptional changes in response to maltose hyperaccumulation stress in chloroplasts or chloroplast damage caused by high light in Arabidopsis leaves.
The ubiquitin-binding NBR1 autophagy receptor plays a prominent role in recognizing ubiquitylated protein aggregates for vacuolar degradation during macroautophagy. Here, we show that upon exposing Arabidopsis plants to intense light, NBR1 associates with photodamaged chloroplasts independently of A...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-01-16 | PXD039183 | Pride
Understanding how cells mitigate lysosomal damage is critical for unraveling pathogenic mechanisms of lysosome related diseases. Here we use organelle-specific proteomics in iPSC derived neurons (i3Neuron) and an in vitro lysosome damaging assay to demonstrate that lysosome damage, caused by the ag...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-11 | MSV000096646 | MassIVE
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