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The proteasome is central to proteolysis by the ubiquitin-proteasome system under normal growth conditions but is itself degraded through macroautophagy under nutrient stress. A recently described AMPactivated protein kinase (AMPK)-regulated endosomal sorting complex required for transport (ESCRT)-d...
ORGANISM(S): Saccharomyces Cerevisiae 
2022-02-06 | PXD031469 |
Pulldown of an ER localized HPPase to identify putative ER microautophagy receptors.
ORGANISM(S): Zea mays (Maize) 
2022-02-08 | PXD011039 | Pride
Stimulator of interferon genes (STING) is essential for the type I interferon response against a variety of DNA pathogens. Upon emergence of cytosolic DNA, STING translocates from the endoplasmic reticulum (ER) to the Golgi where STING activates the downstream kinase TBK1, then to lysosome through r...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-24 | PXD039411 | Pride
To identify the late endosome LE/MVB proteome related to the endosomal microautophagy eMI activity we purified subcellular organelles including cytosol, late endosomes (LE/MVBs) and lysosomes (CMA+) from the rat liver and performed co-Ip experiment with antibodies directed against the major cellular...
ORGANISM(S): Rattus norvegicus (Rat) 
2024-01-26 | PXD046797 | Pride
HeLa cells were transfected with STK38 siRNA or Luc siRNA and then treated with EBSS or LLOMe in three biological replicates. The cells were lysed in 500 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (330...
ORGANISM(S): Homo Sapiens (human) 
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