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MetabolomicsMechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a multi-omi...
2024-08-08 | MTBLS7424 | MetaboLights
MetabolomicsWhat CHO is made of: Variations in the biomass composition of Chinese hamster ovary cell lines

Background: Cell line specific, genome-scale metabolic models enable rigorous and systematic in silico investigation of cellular metabolism. Such models have recently become available for Chinese hamster ovary (CHO) too. However, a key ingredient, namely an experimentally validat...

2020-07-13 | MTBLS1210 | MetaboLights
MetabolomicsAn LC–MS-based lipidomics pre-processing framework underpins rapid hypothesis generation towards CHO systems biotechnology

INTRODUCTION: Given a raw LC–MS dataset, it is often required to rapidly generate initial hypotheses, in conjunction with other ‘omics’ datasets, without time-consuming lipid verifications. Furthermore, for meta-analysis of many datasets, it may be impractical to conduct exhausti...

2018-07-17 | MTBLS493 | MetaboLights
TranscriptomicsCHO smallRNA sequencing for microRNA annotation
MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from CHO cells provide new tools for the discovery of novel miRNAs in this important production system. The current release of the miRNA re...
ORGANISM(S): Cricetulus griseus 
2015-03-02 | E-GEOD-59838 | biostudies-arrayexpress
TranscriptomicsCHO cells with enhanced and abolished gamma-secretase activity
γ-secretase is an intra-membrane-cleaving aspartyl protease implicated in the processing of a wide range of type I membrane proteins including the Notch receptor and the amyloid-β precursor protein (APP). It thus regulates a diverse array of cellular and biological processes including the differen...
ORGANISM(S): Cricetulus griseus 
2010-05-15 | E-GEOD-16379 | biostudies-arrayexpress
TranscriptomicsRevealing suspension adapation of CHO cell in serum free media
Purpose : We performed strand-specific RNA-seq analysis of CHO cells according to adaptation trajectory to identify the specific genes responded to the suspension adaptation. Result : Cell growth, replication, and nucleotide metabolism categories were enriched in up regulated clusters whereas cellul...
ORGANISM(S): Cricetulus griseus 
2015-11-19 | E-GEOD-75094 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of CHO Chr6 cell line
Aim is to validate content of human genomic material in CHO cells monosomal for human chromosome 6. Comparative genomic hybridization to define human genomic regions maintained in hamster hybridoma cell line.
ORGANISM(S): Cricetulus griseus 
2014-06-05 | E-GEOD-56334 | biostudies-arrayexpress
GenomicsCricetulus griseus strain:CHO-K1
CHO cells
PRJDB4351 | ENA
TranscriptomicsGene expression profiles in semi-continuous culture with high-cell density under normal and low temperature conditions for scFv-Fc-producer CHO cells harboring an artificial promoter system with transcriptional positive-feedback loop of transactivator
We developed an artificial gene expression system with transcriptional positive-feedback loop of transactivator to induce the high expression of target genes in CHO cells. DNA microarray analysis was conducted in semi-continuous culture with high-cell-density culture under normal and low temperature...
ORGANISM(S): Cricetulus griseus 
2024-01-01 | E-MTAB-13440 | biostudies-arrayexpress
ProteomicsSWATH-MS insights on butyric acid effect on mAbs production and redox homeostasis in CHO cells.
Sodium butyrate (NaBu) is well-known for its capacity to hinder cellular growth and act as a histone deacetylase inhibitor. It is commonly employed in the cultivation of recombinant Chinese hamster ovary (CHO) cell cultures to boost the production of specific proteins, such as antibodies. In this in...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-05-06 | PXD048575 | Pride
Lc-msms Redox Swath-ms Cho cells Mabs production Butyric acid 
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