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GenomicsThe nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 [HiSeq]
We use a metabolic labeling strategy for directly measuring nucleosome turnover to determine the role of chromatin remodeler Chd1 on chromatin dynamics in mouse embryonic fibroblasts expressing with wildtype-Chd1 and dominant negative K510R-Chd1. We find that expression of K5 10R-Chd1 decreases nucl...
ORGANISM(S): Mus musculus 
2014-04-15 | E-GEOD-52501 | biostudies-arrayexpress
TranscriptomicsMultiple Overlapping Mammalian Chromatin Remodeling Systems Collaborate Genome-wide at Dynamic Chromatin Transitions [Affymetrix]
Expression data from 3134 mouse mammary epithelial cell line -/+ Tet-inducible chromatin remodeler mutant variants ATP-dependent chromatin remodeling is an essential process required for the dynamic organization of chromatin structure. Here we describe the genome-wide location and activity of three...
ORGANISM(S): Mus musculus 
2015-03-05 | E-GEOD-66544 | biostudies-arrayexpress
GenomicsMultiple Overlapping Mammalian Chromatin Remodeling Systems Collaborate Genome-wide at Dynamic Chromatin Transitions (ChIP-Seq)
ATP-dependent chromatin remodeling is an essential process required for the dynamic organization of chromatin structure. Here we describe the genome-wide location and activity of three remodeler proteins with diverse physiological functions in the mouse genome: Brg1, Chd4, and Snf2h. The localizatio...
ORGANISM(S): Mus musculus 
2013-12-24 | E-GEOD-53583 | biostudies-arrayexpress
GenomicsMultiple Overlapping Mammalian Chromatin Remodeling Systems Collaborate Genome-wide at Dynamic Chromatin Transitions (Dnase-Seq)
ATP-dependent chromatin remodeling is an essential process required for the dynamic organization of chromatin structure. Here we describe the genome-wide location and activity of three remodeler proteins with diverse physiological functions in the mouse genome: Brg1, Chd4, and Snf2h. The localizatio...
ORGANISM(S): Mus musculus 
2013-12-24 | E-GEOD-53584 | biostudies-arrayexpress
GenomicsDynamics of yeast ISWI and CHD chromatin remodeler genomic association (part 1)
Chromatin remodelers influence genetic processes by altering nucleosome occupancy, positioning, and composition. In vitro, yeast ISWI and CHD remodelers require > 20 bp of extranucleosomal DNA for remodeling, but linker DNA in S. cerevisiae averages < 20 bp. To resolve this paradox, we have mapped t...
ORGANISM(S): Saccharomyces cerevisiae 
2013-03-01 | E-GEOD-39430 | biostudies-arrayexpress
TranscriptomicsThe FUN30 Chromatin Remodeler Fft3 Protects Centromeric and Subtelomeric Domains from Euchromatin Formation
The chromosomes of eukaryotes are organized into structurally and functionally discrete domains. This implies the presence of insulator elements that separate adjacent domains, allowing them to maintain different chromatin structures. We show that the Fun30 chromatin remodeler, Fft3, is essential fo...
ORGANISM(S): Schizosaccharomyces pombe 
2011-04-02 | E-MEXP-3044 | biostudies-arrayexpress
GenomicsDynamics of yeast ISWI and CHD chromatin remodeler genomic association (part 2)
Chromatin remodelers influence genetic processes by altering nucleosome occupancy, positioning, and composition. In vitro, yeast ISWI and CHD remodelers require > 20 bp of extranucleosomal DNA for remodeling, but linker DNA in S. cerevisiae averages < 20 bp. To resolve this paradox, we have mapped t...
ORGANISM(S): Saccharomyces cerevisiae 
2013-03-01 | E-GEOD-39431 | biostudies-arrayexpress
ProteomicsNucleosome recognition and DNA distortion by the Chd1 remodeler revealed at 2.6 Å resolution
Chromatin remodelers are ATP-dependent enzymes that reorganize nucleosomes within all eukaryotic genomes. The Chd1 remodeler specializes in shifting nucleosomes into evenly spaced arrays, a defining characteristic of chromatin in gene bodies that blocks spurious transcription initiation. Linked to s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Xenopus laevis (African clawed frog) 
2022-02-23 | PXD025287 | Pride
Chd1 Nucleosome Xl-ms Chromatin remodeler 
ProteomicsAffinity Purification of ISWI chromatin remodeler in Trypanosoma cruzi
Chromatin remodeling is a general mechanism involved in DNA-dependent processes, including gene expression regulation. ISWI ATPase is the catalytic subunit in diverse chromatin remodeling complexes that changing histone–DNA interactions and therefore play a pivotal role in different nuclear processe...
ORGANISM(S): Trypanosoma cruzi Dm28c 
2020-05-22 | PXD017869 | Pride
Affinity purification Iswi Trypanosoma cruzi Lc-ms/ms 
ProteomicsThe nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h
Domain level interactions between the ISWI remodeler SNF2h and reconstituted mononucleosomes were probed by EDC crosslinking under different biochemical conditions: ADP, ADP-BeFx, ADP-BeFx with LANA peptide added (which binds at the acidic patch). A semi quantitative approach was used to monitor do...
ORGANISM(S): Xenopus Laevis (ncbitaxon:8355) Homo Sapiens (ncbitaxon:9606) 
2018-03-07 | MSV000082136 | MassIVE
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