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ProteomicsC1QBP mitochondrial interactome
The study of protein-protein interactions is an essential process to understand the biological functions of proteins and the underlying mechanisms. Co-immunoprecipitation coupled with mass spectrometry (CoIP-MS) is one of the most extensively used high-throughput techniques to discover novel protein...
ORGANISM(S): Homo Sapiens 
2015-11-04 | MSV000079369 | MassIVE
TranscriptomicsIdentification of RNA molecules associated with the RNA-chaperone Hfq
We used high-throughput methods to discover the Salmonella RNAs that are targeted by the bacterial Sm-like protein, Hfq. Our generic approach, using chromosomal epitope tagging and coIP-on-chip will also be useful to identify the post-transcriptional regulons of many other RNA-binding proteins. Key...
ORGANISM(S): Salmonella enterica 
2010-06-20 | E-GEOD-10149 | biostudies-arrayexpress
TranscriptomicsIdentification of Hfq bound RNA by co-immunoprecipitation and RNA-sequencing
Co-immunoprecipitation with endogenous Hfq3xFlag in exponential, stationary, non-induced and virulence induced conditions, followed by RNA-sequencing, revealed 1697 mRNAs and 208 ncRNAs associated with Hfq. We identified 56 new ncRNAs and validated 3 Hfq-dependent trans sRNAs on by Northern blot ana...
ORGANISM(S): Agrobacterium tumefaciens 
2014-12-04 | E-GEOD-59123 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of yeast and CoIP-chip of nucleosome-enriched chromatin after immunoprecipitation for specific factors
ChIP-chip & CoIP-chip of nucleosome enriched chromatin after IP of specific factors
ORGANISM(S): Saccharomyces cerevisiae 
2009-09-25 | E-MEXP-1847 | biostudies-arrayexpress
ProteomicsProtein co-immmunoprecipitation using antibodies specific for the antimicrobial peptide FsPDF2 from Fagus sylvatica
To assess the specificity of the antibodies used in the quantitative sandwich ELISA, co-immunoprecipitation (CoIP) was performed. Proteins binding to these antibodies were detected using LC-MS/MS in DDA mode.
ORGANISM(S): Fagus sylvatica 
2026-06-08 | PXD060631 | Pride
Co- immunoprecipitation (coip) Antimicrobial peptides Fagus sylvatica 
UnknownAssessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation.
Not available
S-EPMC7842838 | biostudies-literature
ProteomicsCo-Immunoprecipitation of TMEM65
Mitochondrial Ca2+ transport is a critical process in cellular physiology. One pathway for export occurs via Na+/Ca2+exchange (mito-NCX) machinery. TMEM65 has recently been shown to be crucial for mito-NCX activity, but its precise mechanistic role remains unknown. To evaluate whether TMEM65 forms a...
ORGANISM(S): Homo sapiens (Human) 
2026-02-09 | PXD064897 | Pride
Co-ip Lc-ms/ms 
ProteomicsA0913 interacts with CP47 of PSII and ApcF of PBS
Co-immunoprecipitation (CoIP) was performed to identify the proteins that A0913 interacts with. The thylakoid membranes were solubilized with the detergent n-Dodecyl-β-D-maltoside (DDM) before CoIP was performed using the monoclonal antibodies against the Flag tag to precipitate the target proteins....
ORGANISM(S): Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum) 
2024-11-06 | PXD057430 | Pride
Co-ip 0913-cf Wt Lc-msms Cp47 
ProteomicsIdentification of GltB-interacting proteins by CoIP-MS
To investigate whether GltB possess more function, Co-immunoprecipitation coupled with Mass Spectrometry (CoIP-MS) was used to isolate the putative GltB binding proteins.We used strains PAO1/p-gtrS-YFP and ΔgltB/p-gtrS-YFP to ensure the membrane protein GtrS of great interest is at high levels. A ta...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2021-09-09 | PXD020213 | Pride
Pseudomonas aeruginosa Gltb-interacting protein Lc-ms/ms 
TranscriptomicsIdentification of StpA-binding sites on the Salmonella genome
We used ChIP-chip analysis to identify where the nucleoid associated protein StpA binds on the Salmonella Typhimurium genome. We identified 285 chromosomal regions bound by StpA, ranging in size from 400 to 3500 base pairs and containing 572 genes. Comparison of the ChIP data revealed that StpA disp...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 
2009-10-28 | E-GEOD-18450 | biostudies-arrayexpress
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