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TranscriptomicsDisplacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting
The ring-shaped cohesin complex links sister chromatids until their timely segregation during mitosis. Cohesin is enriched at centromeres, where it provides the cohesive counter-force to bi-polar tension produced by the mitotic spindle. As a consequence of spindle tension, centromeric sequences tran...
ORGANISM(S): Saccharomyces cerevisiae 
2007-09-29 | E-GEOD-8661 | biostudies-arrayexpress
TranscriptomicsCell-cycle regulation of cohesin stability along fission yeast chromosomes
Sister chromatid cohesion is mediated by cohesin but the process of cohesion establishment during S phase is still enigmatic. Recent data indicate that in mammalian cells, cohesin binding to chromatin is dynamic in G1 but becomes stabilized during S phase. Whether the regulation of chromosomal cohes...
ORGANISM(S): Schizosaccharomyces pombe 
2007-11-30 | E-GEOD-8450 | biostudies-arrayexpress
ProteomicsStructure of the Cohesin Loader Scc2-Scc4 and its Binding to a Folded Cohesin
The functions of cohesin are central to genome integrity, chromosome organization, and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex, however, little is k...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-01-11 | PXD004692 | Pride
Cohesin Cross-linking Cx-ms 
ProteomicsCrosslinking/mass spectrometry (CLMS) analysis of Cohesin complex
The ring-shaped cohesin complex is thought to fulfil its roles in sister chromatid cohesion, genome stability and gene regulation by topologically encircling DNAs. The ring is formed by two Structural Maintenance of Chromosome (SMC) subunits, whose ATPase heads are linked by a kleisin subunit. Addit...
ORGANISM(S): Schizosaccharomyces pombe 972h- 
2020-08-04 | PXD018608 | Pride
Crosslinking/mass spectrometry Cohesin complex 
TranscriptomicsBudding yeast cohesin (Mcd1) distribution in mitotically arrested cells
genomic localization of the budding yeast cohesin complex was mapped in mitotically arrested cells by Mcd1 ChIP followed by hybridization to high-density tiled microarrays Cells were synchronized first in G1 and then released into media containing the microtubule poison nocodazole. Cells were fixed ...
ORGANISM(S): Saccharomyces cerevisiae 
2009-09-30 | E-GEOD-18021 | biostudies-arrayexpress
TranscriptomicsGenome-wide control of RNA polymerase II activity by cohesin (sequencing)
Cohesin is a well-known mediator of sister chromatid cohesion, but it also influences gene expression and development. These non-canonical roles of cohesin are not well understood, but are vital: gene expression and development are altered by modest changes in cohesin function that do not disrupt ch...
ORGANISM(S): Drosophila melanogaster 
2013-04-24 | E-GEOD-42397 | biostudies-arrayexpress
TranscriptomicsGenome-wide control of RNA polymerase II activity by cohesin (tiling)
Cohesin is a well-known mediator of sister chromatid cohesion, but it also influences gene expression and development. These non-canonical roles of cohesin are not well understood, but are vital: gene expression and development are altered by modest changes in cohesin function that do not disrupt ch...
ORGANISM(S): Drosophila melanogaster 
2013-04-24 | E-GEOD-42360 | biostudies-arrayexpress
ProteomicsLC/MS Analysis of Myc-Affinity Captured, on-Beads Crosslinked, and on-Beads Digested S. cerevisiae SCC2 subunit of cohesin loading factor
Myc-Affinity Capture and on-Beads Crosslinking For each Saccharomyces cerevisiae strain (C-terminally myc-tagged Scc2 subunit of the cohesin loading factor complex or wild-type BY4741), 2x 3 L YPD medium (add 300 ml 20% Glucose to each 3 L of YPD before using) were separately inoculated in a 5L flas...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-10-20 | MSV000086339 | MassIVE
TranscriptomicsA Myc-dependent role for cohesin in the heterokaryon-mediated reprogramming of somatic nuclei
Analysis of gene expression changes in mESCs where the cohesin subunit Scc1 has been depleted. There are 6 samples. 3 Scc1 wt/wt ERT2-Cre biological replicas and 3 Scc1 lox/lox ERT2-Cre biological replicas, all treated with 4'-OHT 80nM final concentration for 24h. Scc1 wt/wt and Scc1 lox/lox ERT2-Cr...
ORGANISM(S): Mus musculus 
2015-02-19 | E-GEOD-62325 | biostudies-arrayexpress
ProteomicsAbsolute quantification of cohesin and its regulators in human cells
The organisation of mammalian genomes into loops and topologically associated domains (TADs) regulates gene expression. The formation and maintenance of loops and TADs depends on the protein complex cohesin, which also holds replicated sister chromatids together from S phase until mitosis. To unders...
ORGANISM(S): Homo sapiens (Human) 
2019-11-13 | PXD012712 | Pride
Fluorescence-correlation spectroscopy Mass spectrometry Cell cycle Genome organization Cohesin Sister chromatid cohesion 
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