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Identification of gene expressed in the enriched inner medullary collecting duct cells in rat. Experiment Overall Design: Rat inner medullary collecting duct (IMCD) cells were isolated from 7 male Sprage-Dawley rats by collagenase and hyaluronidase digestion and follow by low speed centrifugation. T...
ORGANISM(S): Rattus norvegicus 
Kidneys are essential for acid-base homeostasis, especially when organisms cope with changes in acid or base dietary intake. Because collecting ducts constitute the final site for regulating urine acid-base balance, we undertook to identify the gene network involved in acid-base transport and regula...
ORGANISM(S): Mus musculus 
We would like to know the gene expression pattern in absence of transcription factor GATA2 in adult renal collecting duct We used Gata2 flox::Pax8-rtTA::Tet-Cre to make a doxycycline induced Gata2 renal tubule cell specific knockout mice We performed microarray analyses using DBA-lectin and magnetic...
ORGANISM(S): Mus musculus 
Libraries obtained from microdissected kidney outer medullary collecting duct from C57Bl6/J mice control or after 3 days potassium-depleted diet. Keywords: other
ORGANISM(S): Mus musculus 
We profiled SUMO1 and SUMO2 modified proteins in cortical collecting duct (mpkCCD) as a precursor to targeted approaches to assess SUMO function. SUMO levels in cells following heat shock and treatment with deSUMOylation inhibitors were assessed.
ORGANISM(S): Mus musculus (Mouse) 
2019-10-07 | PXD013346 | Pride
Murine inner medullary collecting duct cells were treated for 1 hour with vehicle (control) or aldosterone. Total RNA was isolated and used as template to generate the eventual cRNA target. The experiment was repeated a total of three times. Six cRNA samples, three control and three treated, were ge...
ORGANISM(S): Mus musculus 
SILAC was employed to investigate the effect of aldosterone stimulation on SUMOylation of cortical collecting duct (mpkCCD) cells.
ORGANISM(S): Mus musculus (Mouse) 
2019-10-07 | PXD013363 | Pride
Phosphorylation of the aquaporin-2 (AQP2) water channel at four COOH-terminal serines plays a central role in the regulation of water permeability of the renal collecting duct. The level of phosphorylation at these sites is determined by a balance between phosphorylation by protein kinases and depho...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-10-26 | PXD005488 | Pride
Data from ProteomeXchange, PXD ID: PXD002434. File: 335.mzml. Published as part of Am J Physiol Cell Physiol. 2015 Aug 26:ajpcell.00214.2015 and Am J Physiol Cell Physiol. 2015 Aug 26:ajpcell.00213.2015
ORGANISM(S): Mus_musculus_viruses, Mouse 
Response of collecting duct cells to hypertonic stress
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