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To elucidate the early regulators of inflammation during Mycobacterium tuberculosis infection, we performed a genome-wide CRISPR knockout screen in macrophages to identify genes that influence the induction of TNF and iNOS upon infection. A genome-wide knockout library in murine macrophages was infe...
ORGANISM(S): Mus musculus 
To identify genes involved in macrophage differentiation, a genome-wide CRISPR knockout library in macrophage progenitors was differentiated into macrophages. Samples were collected from the progenitor library at day 0, from a library maintained as progenitors for seven days, and from macrophages af...
ORGANISM(S): Mus musculus 
RNA-seq profiling of macrophages lacking Irf2, Irf9, or Irf2 and Irf9, as well as scramble controls was performed. Macrophages were infected with Listeria monocytogenes or uninfected under mock conditions and total RNA was harvested 2 hours post infection.
ORGANISM(S): Mus musculus 
We applied TurboID-based proximity labelling restricted to Advillin-expressing neurons to systematically profile the proteomes of distinct neuronal subcompartments, using parallel accumulation–serial fragmentation combined with data-independent acquisition (diaPASEF) mass spectrometry. Neuronal comp...
ORGANISM(S): Mus musculus (Mouse) 
2026-04-14 | PXD062198 | Pride
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