Newly synthesized proteome analysis of EPP2 murine pancreatic cancer cells with induced knockout of tRNA wobble enzymes Elp3 or Ctu1 and mTOR inhibitor treatment. Knockouts were induced for 4 days, mTOR inhibitors were 5 h rapamycin [50 nM] or torin 1 [50 nM].
Quantitative and qualitative changes in mRNA translation occur in tumor cells and support cancer progression and metastasis. Post-transcriptional nucleoside modifications of transfer RNAs (tRNAs) at the wobble U34 base are highly conserved and contribute to translation fidelity. Here, we show that E...
Synthesizing the cellular proteome is a demanding process that is regulated by numerous signaling pathways and RNA modifications. How these orchestrate the protein synthesis machinery to generate specific proteome subsets remains unclear. We found when mTORC1 was inactive, tRNA wobble uridine-modify...
