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Metabolomics4-Octyl itaconate as a metabolite derivative inhibits inflammation via alkylation of STING

The Krebs cycle-derived metabolite itaconate, whose production is catalyzed by immune response gene 1 (IRG1), has excellent potential to link immunity and metabolism in activated macrophages through alkylation or competitive inhibition of target proteins. In support of this, our previous study de...

2023-01-15 | MTBLS5877 | MetaboLights
Proteomics‘Shotgun’ Proteome Analyses Do Not Require Alkylation of Cysteine
It is a common belief that reduction of disulfide bridges and alkylation of thiols in proteins are indispensable steps in proteomic sample preparation. Since this chemical procedure is often incomplete and prone to side reactions and can results in various artefacts we reexamined its importance. We ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-21 | PXD014000 | Pride
Thiol alkylation Iodoacetamide Shotgun proteomics 
ProteomicsCysteine alkylating methods in shotgun proteomics and their possible effects on methionine residues
Neatly every proteomic protocol has a Cysteine reduction and alkylation step in it preceding the trypsin digestion. It needs to be done in order to remove the s-s bonds and prevent proteins from folding, thus, provide better access for trypsin. Among various reagents used for alkylation, iodoacetami...
ORGANISM(S): Homo sapiens (Human) 
2020-10-28 | PXD013973 | Pride
Cysteine Proteomics Alkylation Methionine 
ProteomicsCysteine alkylating methods in shotgun proteomics and their possible effects on methionine residues
Neatly every proteomic protocol has a Cysteine reduction and alkylation step in it preceding the trypsin digestion. It needs to be done in order to remove the s-s bonds and prevent proteins from folding, thus, provide better access for trypsin. Among various reagents used for alkylation, iodoacetami...
ORGANISM(S): Homo sapiens (Human) 
2024-02-14 | PXD015889 | Pride
Cysteine Proteomics Alkylation Methionine Reduction 
TranscriptomicsThe global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA)
Abstract of associated menuscript; Quinones and alpha,beta-unsaturated carbonyls are naturally occurring electrophiles that target cysteine residues via thiol-(S)-alkylation. We analyzed the global expression profile of Bacillus subtilis to the toxic carbonyls methylglyoxal (MG) and formaldehyde (FA...
ORGANISM(S): Bacillus subtilis 
2010-05-15 | E-GEOD-13925 | biostudies-arrayexpress
ProteomicsSupporting information for tryptic EPO glycopeptide LC-MS analysis via cysteine aminoethylation
Supplementary information for a technical note describing the application of cysteine aminoethylation for the site-specific glycosylation analysis of recombinant human EPO using trypsin as sole protease.
ORGANISM(S): Cho Cells 
2020-04-21 | MSV000085318 | MassIVE
ProteomicsSystematic Evaluation of Protein Reduction and Alkylation reveals Massive Unspecific Side Effects by Iodine-containing Reagents
In this study, we systematically evaluated reduction and alkylation of proteins using three reducing agents (DTT, TCEP, and BME) in combination with four alkylation agents (IAA, IAC, AA, and CAA). We tested these conditions using strong anion exchange (SAX) fractionated in-solution digests and in-ge...
ORGANISM(S): Homo sapiens (Human) 
2017-05-30 | PXD005183 | Pride
Alkylation Proteomics Reduction 
ProteomicsAlkylation Damage by Lipid Electrophiles-- HNE-induced expression in RKO cells
4-hydroxynonenal (HNE) (UniMod 53) (+156.115030 Da on Cys, His, Lys) reduced 4-Hydroxynonenal (UniMod 335(+158.130680 Da on Cys, His, Lys) These data were additions to the set for the paper Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems by Codreanu et al, Mol. Cell. Pro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2013-12-18 | MSV000078512 | MassIVE
ProteomicsIdentification of cysteine redox states in MTP from PDI-deficient mouse liver
Cysteine redox states in MTP from PDI-deficient mouse liver were determined using differential cysteine alkylation and PRM-MS analysis.
ORGANISM(S): Mus musculus (Mouse) 
2025-01-16 | PXD055650 | Pride
Mtp Differential cysteine alkylation Prm-ms Redox state of cysteines 
ProteomicsThe impact of commonly used alkylating agents on artefactual peptide modification
Iodoacetamide is by far the most commonly used agent for alkylation of cysteine during sample preparation for proteomics. An alternative, 2-chloroacetamide, has been recently suggested to reduce the alkylation of residues other than cysteine, such as the N-terminus, Asp, Glu, Lys, Ser, Thr and Tyr....
ORGANISM(S): Rattus norvegicus (Rat) 
2017-08-16 | PXD007071 | Pride
Post-translational modification Cysteine alkylation Artefact N-terminal alkylation Alkylation 
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