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MetabolomicsGlutathione is a host-derived cysteine source for Clostridioides difficile
Clostridioides difficile is the leading cause of nosocomial diarrhea, afflicting approximately half a million people each year in the USA, burdening both individuals’ quality of life and the healthcare system. In the gastrointestinal (GI) tract, C. difficile must acquire essential nutrients to colon...
2026-06-10 | MTBLS14740 | MetaboLights
MetabolomicsIsotope Labeled Reactivity-Based Metabolomics Reveal Cysteine as a Potent In Vivo Scavenger of Lactoylglutathione

Metabolite adducts are increasingly being recognized as important intermediates in cellular metabolism and as circulating messengers. Methylglyoxal (MG) is a highly reactive metabolite involved in diabetes and aging, via formation of a wide variety of protein adducts. Though new types of protein ...

2025-04-29 | MTBLS6287 | MetaboLights
TranscriptomicsTranscription profiling of human liver cell line after cysteine deprivation
First experiment: Cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM methionine + 0.1 mM cysteine (complete) or supplemented only with 0.1 mM methionine (cysteine-free). Cells were cultured in either medium for 42 h (Long + Cys; Long -Cys) or in cysteine-free medium for 36 ...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-9517 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human N-Acetyl L-cysteine induced differentiation of NHEK cells in vitro
Gene expression analysis of N-Acetyl L-cysteine induced differentiation of NHEK cells in vitro.
ORGANISM(S): Homo sapiens 
2005-11-30 | E-MEXP-500 | biostudies-arrayexpress
Transcriptomicsglobal gene expression profiling experiments of HSC cultured in cysteine or valine deficient medium by RNA-sequencing analysis
We investigated the role of amino acid to maintain HSC function. To identify essential amino acids for HSCs, CD34-KSL cells were cultured in single amino acids deficient medium. And cultured cells were transplanted into lethally irradiated mice. Then, the donor chimerism and lineage contribution was...
ORGANISM(S): Mus musculus 
2016-06-26 | E-GEOD-70307 | biostudies-arrayexpress
MetabolomicsMechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a multi-omi...
2024-08-08 | MTBLS7424 | MetaboLights
ProteomicsGlobal profiling of phosphorylation-dependent changes in cysteine reactivity
Proteomics has revealed that the ~20,000 human genes engender a far greater number of proteins, or proteoforms, that are diversified in large part by post-translational modifications (PTMs). How such PTMs affect protein structure and function is an active area of research but remains technically cha...
ORGANISM(S): Homo sapiens (Human) 
2022-03-03 | PXD026730 | Pride
Cysteine Activity-based protein profiling Tmt-abpp Mitosis Chemical proteomics Phosphorylation 
ProteomicsNAIA for cysteine profiling in HepG2 cells, protein target identification of covalent ligand and studying cysteine oxidative modifications
New cysteine probes, N-acryloylindole-alkynes (NAIAs), have been developed. We have applied NAIA-5 for cysteine profiling in HepG2 cell lysates, and the results have been compared to the conventional cysteine probe iodoacetamide-alkyne (IAA). NAIA-5 allows identification of more ligandable Cys than ...
ORGANISM(S): Homo sapiens (Human) 
2023-06-14 | PXD041264 | Pride
Profiling ligandable cysteines and studying cysteine oxidative modifications in human liver cancer cells hepg2 by naia-5 using bruker timstof ms 
ProteomicsProteomic Cysteine Profiling of DMI- and DMF-treated MEFs
Dimethyl fumarate (DMF) and dimethyl itaconate (DMI) are electrophilic metabolite derivatives with potent immunomodulatory properties. While DMF is clinically approved for multiple sclerosis, the molecular mechanisms underlying the actions of both compounds remain incompletely understood. Both have ...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-18 | PXD080692 | Pride
Mouse Cysteine reactivity profiling Mef Abpp 
TranscriptomicsExogenous Sulfide Reverses the Alteration of Transcriptional Profiling of the des1-1 Mutant (Arabidopsis thaliana)
Arabidopsis thaliana cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes that catalyze the biosynthesis of cysteine. Recently, we have deeply investigated about one of the minor OASTL-like protein located in the cytosol, named DES1, highlighting some important clues about its metaboli...
ORGANISM(S): Arabidopsis thaliana 
2013-04-25 | E-GEOD-32566 | biostudies-arrayexpress
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