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MetabolomicsIsotope Labeled Reactivity-Based Metabolomics Reveal Cysteine as a Potent In Vivo Scavenger of Lactoylglutathione

Metabolite adducts are increasingly being recognized as important intermediates in cellular metabolism and as circulating messengers. Methylglyoxal (MG) is a highly reactive metabolite involved in diabetes and aging, via formation of a wide variety of protein adducts. Though new types of protein ...

2025-04-29 | MTBLS6287 | MetaboLights
ProteomicsHeterogeneous adaptation of cysteine reactivity to a covalent oncometabolite
Metabolism and signaling intersect in the genetic cancer syndrome hereditary leiomyomatosis and renal cell carcinoma (HLRCC), a disease in which mutation of the TCA cycle enzyme fumarate hydratase (FH) causes hyperaccumulation of fumarate. This electrophilic oncometabolite can alter gene activity at...
ORGANISM(S): Homo sapiens (Human) 
2021-05-06 | PXD020073 | Pride
Human Cysteine reactivity Kidney Hlrcc Uok268 
ProteomicsGlobal profiling of phosphorylation-dependent changes in cysteine reactivity
Proteomics has revealed that the ~20,000 human genes engender a far greater number of proteins, or proteoforms, that are diversified in large part by post-translational modifications (PTMs). How such PTMs affect protein structure and function is an active area of research but remains technically cha...
ORGANISM(S): Homo sapiens (Human) 
2022-03-03 | PXD026730 | Pride
Cysteine Activity-based protein profiling Tmt-abpp Mitosis Chemical proteomics Phosphorylation 
ProteomicsCIAA: Integrated Proteomics and Structural Modeling for Understanding Cysteine Reactivity with Iodoacetamide Alkyne
This study utilizes cysteine chemoproteomics paired with a high-throughput structure-guided in silico approach towards assessing structural motifs of cysteines that are highly reactive towards iodoacetamide alkyne (IAA).
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD056064 | Pride
Reactivity Cysteine Chemoproteomics Isotop-abpp 
ProteomicsCysteine reactivity profiling identifies host regulators of Mycobacterium tuberculosis replication in human macrophages
Innate immune cells such as monocytes and macrophages provide the earliest defense against infection by intracellular pathogens by initiating signaling pathways and restricting pathogen replication. However, the full complement of proteins that mediate cell-autonomous immunity remains incompletely d...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD067709 | Pride
Innate immunity Cysteine Macrophage Mycobacterium tuberculosis Mass spectrometry Chemical proteomics Covalent probes 
TranscriptomicsAn O-acetylserine(thiol)lyase homolog regulates cysteine homeostasis in Arabidopsis thaliana
Cysteine occupies a central position in plant metabolism due to its biochemical functions. Arabidopsis thaliana cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes that catalyze the biosynthesis of cysteine. Because they are localized in the cytosol, plastids and mitochondria, this re...
ORGANISM(S): Arabidopsis thaliana 
2011-11-23 | E-GEOD-19244 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human liver cell line after cysteine deprivation
First experiment: Cells were cultured in sulfur amino acid-free DMEM supplemented with 0.1 mM methionine + 0.1 mM cysteine (complete) or supplemented only with 0.1 mM methionine (cysteine-free). Cells were cultured in either medium for 42 h (Long + Cys; Long -Cys) or in cysteine-free medium for 36 ...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-9517 | biostudies-arrayexpress
ProteomicsChemoselective umpolung of thiols to episulfoniums for cysteine bioconjugation
Modification of native cysteine is an important tool in protein research and relies on fast and chemoselective reactions under bioambient conditions. Nucleophilic cysteine can be functionalized with electrophiles, such as maleimides, that carry specific chemical groups, depending on the desired appl...
ORGANISM(S): Escherichia Coli 
2023-12-20 | PXD038723 | JPOST Repository
TranscriptomicsTranscription profiling of human N-Acetyl L-cysteine induced differentiation of NHEK cells in vitro
Gene expression analysis of N-Acetyl L-cysteine induced differentiation of NHEK cells in vitro.
ORGANISM(S): Homo sapiens 
2005-11-30 | E-MEXP-500 | biostudies-arrayexpress
ProteomicsSLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis
This study investigates how loss of the ER transporter SLC33A1 affects glutathione redox balance and protein cysteine reactivity in the endoplasmic reticulum. Oxidized cysteine reactivity profiling and whole-cell proteomics were performed in Slc33a1 knockout KPK cells and cDNA-complemented controls....
ORGANISM(S): Mus musculus (Mouse) 
2026-02-13 | PXD074382 | Pride
Glutathione Tmt proteomics Er redox Slc33a1 Cysteine reactivity Mouse cells Lc–ms/ms 
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