This study characterizes the metabolome of the Medicago truncatula-Sinorhizobium meliloti symbiosis. Symbiosome space, bacteroid, and nodule cytosol fractions were isolated from 4-week-old root nodules and profiled by untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). Polar metabo...
The human liver cytosol stability model is used for predicting the stability of a drug in the cytosol of human liver cells, which is beneficial for identifying potential drug candidates early during the drug discovery process. If a drug compound is quickly absorbed, it may not reach the intended tar...
In eukaryotic cells, the spatial regulation of protein expression is frequently conferred through the coupling of mRNA localization and the local control of translation. mRNA localization to the endoplasmic reticulum (ER) is a prominent example of such regulation and serves a ubiquitous role in segr...
The cytoplasm is an essential part of the cell involved in protein synthesis, energy generation, nutrient processing, transport, metabolism, and homeostasis, but its structure and function are not entirely understood. Almost five decades ago, dynamic nano-compartments of the cytoplasm were observed ...
The energetic (ATP) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. Cytosolic acetyl-CoA is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into Saccharo...
PolyA+ RNA from cytosol of HeLa and GM06990 cells using Affymetrix ENCODE tiling chip with NCBI build 35 annotation. PolyA+ RNA from cytosol of HeLa and GM06990 cells.
We report separation of the viscous fluid component of the cytoplasm, the cytosol, from the elastic solid, the cytomatrix and describe distinct molecular pathways of each compartment.
In the past decades many studies reported the presence of Endoplasmic Reticulum (ER) resident proteins in the cytosol. However the mechanisms by which these proteins relocate and whether they exert cytosolic functions remain unknown. We found that select ER luminal proteins accumulate in the cytosol...
