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This study characterizes the metabolome of the Medicago truncatula-Sinorhizobium meliloti symbiosis. Symbiosome space, bacteroid, and nodule cytosol fractions were isolated from 4-week-old root nodules and profiled by untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). Polar metabo...
2026-08-19 | MTBLS15392 | MetaboLights
In eukaryotic cells, the spatial regulation of protein expression is frequently conferred through the coupling of mRNA localization and the local control of translation. mRNA localization to the endoplasmic reticulum (ER) is a prominent example of such regulation and serves a ubiquitous role in segr...
ORGANISM(S): Homo sapiens 
Transcriptome profiling of cytosol, endoplasmic reticulum (ER), and pellet fraction from the Xenopus laevis oocyte and mature egg
Mutations in the DJ-1 (Park7) gene cause autosomal recessive Parkinson's disease in humans, but the function of the DJ-1 protein is poorly characterized. In an effort to understand more about the biology of DJ-1, we performed iTRAQ analyses on subcellular fractions enriched from DJ-1 knockout rat an...
ORGANISM(S): Mus musculus (Mouse) Rattus norvegicus (Rat) 
2017-10-04 | PXD005215 | Pride
In the past decades many studies reported the presence of Endoplasmic Reticulum (ER) resident proteins in the cytosol. However the mechanisms by which these proteins relocate and whether they exert cytosolic functions remain unknown. We found that select ER luminal proteins accumulate in the cytosol...
ORGANISM(S): Homo sapiens (Human) 
2021-05-03 | PXD023567 | Pride
This genome-wide transcriptome analysis was aimed to reveal dynamic regulation of mRNA-ER association during Xenopus oocyte maturation. We extracted RNAs from the cytosolic, ER, and the pellet fraction of oocytes and eggs, respectively, and performed RNA-sequencing. These results showed that mRNA-ER...
ORGANISM(S): Xenopus laevis 
2022-12-13 | GSE199254 | GEO
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