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ProteomicsFAIMS-GPF XL-MS: crosslinking-mass spectrometry based on gas-phase fractionation
Protein-protein interactions (PPIs) underpin nearly all cellular processes; therefore, mapping PPI and protein-protein association networks is critical for understanding how biological systems function in health and disease. However, many functionally relevant PPIs are transient and mediated by weak...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD064228 | Pride
Faims Cross-linking mass spectrometry Structural biology Interactomics 
ProteomicsCirculating tumour cells shed large extracellular vesicles in capillary-scale bifurcations
Data Independant Acquisition (DIA)-based proteomics to characaterise the proteins identified in Large Extracellular vesicles (LEVs) samples.
ORGANISM(S): Homo sapiens (Human) 
2025-09-01 | PXD050444 | Pride
Large extracellular vesicles 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054105 | Pride
Data independant acquistion Label free quantification 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - 80 compound screen (HEK293T and Jurkat)
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054127 | Pride
Chemoproteomics Data independant acquisition Label-free qauntification 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - Library Build, concentration response, structure activity relationships and in-cell treatment
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054145 | Pride
Chemoproteomics. lavel-free quantification Data independant acquisition 
ProteomicsLaser microdissection and proteomic study of paraffin-embedded fetal and paediatric lung tissues.
The goal of this project was to understand if lasermicrodissected epithial portions of postanatal and fetal human lung tissue could be used for proteomics, and then, further confirmed by immunostaining. The proteomic data obtained from postanatal bronchi was compared to the one obtained from alveoli...
ORGANISM(S): Homo sapiens (Human) 
2023-08-14 | PXD040654 | Pride
Ffpe  human lung  fetal tissue  lmd 
ProteomicsNeisseria meningitides small RNA's regulate Citric acid cycle activity under Feast and Famine conditions
We identified twin small non-coding RNAs regulating tricarboxylic acid (TCA) cycle activity in Neisseria meningitidis. Expression of TCA cycle enzymes was elevated in sRNA deletion mutants. Direct interaction between sRNAs and the ribosomal entry sites on target mRNAs was demonstrated. Expression of...
ORGANISM(S): Neisseria meningitidis serogroup B 
2017-03-22 | PXD000891 | Pride
Data independant mass spectrometry Pathogens Neisseria meningitides Feast and famine Small rna's 
ProteomicsProteomics of Campylobacter glycosylation
N-linked glycosylation is an essential virulence determinant in Campylobacter jejuni, the major causative agent of gastroenteritis in the developed world. Glycosylation is encoded by the pgl gene cluster which encodes for the biosynthesis and attachment of a conserved heptasaccharide glycan to prote...
ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 
2019-01-11 | PXD011646 | Pride
Glycosylation Proteomics Tandem mass tag Campylobacter Lc-ms/ms Data independant acquisiton 
ProteomicsProtein content of extracellular vesicles from human brain microvascular endothelial cells treated by paraquat
Since the last decade, the field of extracellular vesicles (EVs) has increasingly grown. These “intracellular messengers” became interesting as biomarker source in the SNC research, because of their content, which may reflect the physiological state of their donor cells in a context of an external s...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD024691 | Pride
Endothelia cells Proteomics Extracellular vesicles Dia-ms Paraquat Cns Ubiquinone metabolism Hif-1 Bbb 
ProteomicsNucleotide independant as well as ATP-hydrolysis driven steps of Rea1 Linker remodelling drive the removal of assembly factors from pre-ribosomal particles: PhoX cross-linking of Rea1 in presence of ATPgS, AMP-PNP or Apo state.
The ribosome maturation factor Rea1 (or Midasin) catalyses the removal of assembly factors from large ribosomal subunit precursors and to promotes their export from the nucleus to the cytosol. Rea1 consists of nearly 5000 amino-acid residues and belongs to the AAA+ protein family. It consists of a r...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-07-29 | PXD053636 | Pride
Cross-linking mass spectrometry Structural biology Structural proteomics 
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