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TranscriptomicsRNAi knock down of mRNA decapping co-activators in Drosophila S2 cells
We analyzed mRNA expression profiles in Drosophila melanogaster S2 cells that had been depleted of proteins known as mRNA decapping co-activators. mRNA decapping is catalyzed by DCP2, and DCP2 activity is stimulated by decapping co-activators. This group of proteins includes DCP1, Hedls (also known ...
ORGANISM(S): Drosophila melanogaster 
2007-09-03 | E-MEXP-1012 | biostudies-arrayexpress
TranscriptomicsNudt3 is a mRNA Decapping Enzyme That Modulates Cell Migration
The Dcp2 and Nudt16 Nudix hydrolases, are mRNA decapping enzymes that preferentially modulate stability of a subset of mRNAs. Here we report Nudt3 is a third Nudix protein that possess mRNA decapping activity in cells and is a modulator of cell migration in MCF-7 breast cancer cells. Genome-wide a...
ORGANISM(S): Homo sapiens 
2016-07-21 | E-GEOD-75146 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis mutant tdt-1 and wild type
The object of this study was to explore whether the loss of mRNA decapping in the Arabidopsis mutant tdt-1 resulted in global dfferences of RNA profiles, as compared to wild type. Experiment Overall Design: In this experiment we compared expression in tdt-1 and wild type 3 day whole seedlings. We p...
ORGANISM(S): Arabidopsis thaliana 
2008-06-15 | E-GEOD-7359 | biostudies-arrayexpress
ProteomicsMolecular connection between the TUTase URT1 and decapping activators
Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/de...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-29 | PXD018672 | Pride
Arabidopsis Tutase Deadenylation Rna degradation Decapping Uridylation 
TranscriptomicsComparison of alternative decapping enzymes to map transcription start sites genome-wide
The production of Tobacco Acid Pyrophosphatase (TAP), an enzyme commonly used for the removal of the 5â??cap of eukaryotic mRNAs, has been recently discontinued. Here we performed a comparison of current alternatives for the mapping of 5â??cap mRNAs and the associated transcription start sites in Sa...
ORGANISM(S): Saccharomyces cerevisiae 
2016-01-06 | E-GEOD-73819 | biostudies-arrayexpress
ProteomicsA unique mRNA decapping complex in trypanosomes
Removal of mRNA 5’ caps primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme DCP2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5´-3´exoribonuclease Xrn1. Kinetoplastida lack DCP2...
ORGANISM(S): Trypanosoma brucei 
2023-06-14 | PXD038550 | Pride
Xrna Mrna decay Apah-like phosphatase Alph1 Mrna decapping Trypanosoma Exoribonuclease 
TranscriptomicsEffects of Dcp1a and Dcp2 knockdown during mouse oocyte maturation
Oocyte maturation is accompanied by a transition from mRNA stability to instability. We investigated the role of DCP1A and DCP2, proteins responsible for mRNA decapping, in mRNA destabilization during mouse oocyte maturation. siRNA-mediated knockdown of both Dcp1a and Dcp2 transcripts prior to ini...
ORGANISM(S): Mus musculus 
2013-02-06 | E-GEOD-27049 | biostudies-arrayexpress
ProteomicsImmunoisolation of the trypanosome decapping enzyme ALPH1 delivers a potential subcomplex
Removal of mRNA 5’ caps primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme DCP2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5´-3´exoribonuclease Xrn1. Kinetoplastida lack DCP2...
ORGANISM(S): Trypanosoma brucei 
2023-06-14 | PXD042322 | Pride
Xrna Mrna decay Apah-like phosphatase Alph1 Mrna decapping Trypanosoma Exoribonuclease 
GenomicsmRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription
We report a function of human mRNA decapping factors in control of transcription by RNA polymerase II. Decapping proteins Edc3, Dcp1a and Dcp2 and the termination factor TTF2 co-immunoprecipitate with Xrn2, the nuclear 5'-3' exonuclease torpedo that facilitates transcription termination at the 3' e...
ORGANISM(S): Homo sapiens 
2012-04-04 | E-GEOD-36185 | biostudies-arrayexpress
TranscriptomicsGlobal analysis of mRNA decay intermediates in Saccharomyces cerevisiae
The general pathways of eukaryotic mRNA decay occur via deadenylation followed by 3’ to 5’ degradation or decapping, although some endonuclease sites have been identified in metazoan mRNAs. To determine the role of endonucleases in mRNA degradation in Saccharomyces cerevisiae, we mapped 5’ monophosp...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-17 | E-GEOD-33712 | biostudies-arrayexpress
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