Sort   by:  
 Page size 
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus musculus (Mouse) 
2019-01-24 | PXD011691 | Pride
Tmt Orbitrap fusion lumos Dia 
ProteomicsA Comparison of Quantitative Mass Spectrometric Methods for Drug Target Identification by Thermal Proteome Profiling
Benchmarking different LFQ-DIA approaches with traditional TMT-DDA for thermal proteome profiling.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD040173 | Pride
Hybrid library Target deconvolution Data-dependent acquisition (dda) Tandem mass tag (tmt) Thermal proteome profiling (tpp) Acute myeloid leukaemia (aml) Spectral library Data-independent acquisition (dia) 
ProteomicsA mouse model of Fragile X Syndrome analyzed by quantitative proteomics: a comparison of methods
We have compared the performance of seven different strategies in the analysis of a mouse model of Fragile X Syndrome, involving the knockout of the fmr1 gene that is the leading cause of autism spectrum disorder. Focusing on the cerebellum, we show that Data-Independent Acquisition (DIA) and the TM...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-21 | PXD039885 | Pride
Quantitative proteomics Boxcar Complementary ion quantification Dia Method comparison Label-free Tmt labeling Mouse brain proteomics Fragile x syndrome 
ProteomicsBenchmarking of quantitative proteomics workflows for Limited proteolysis mass spectrometry
Limited proteolysis coupled with mass spectrometry (LiP-MS) has emerged as a powerful technique for detecting protein structural changes and drug-protein interactions on a proteome-wide scale. However, there is no consensus on the best quantitative proteomics workflow for analyzing LiP-MS data. In t...
ORGANISM(S): Homo sapiens (Human) 
2025-03-31 | PXD055927 | Pride
Dia Tmt Limited proteolysis Staurosporine 
ProteomicsCross-Platform Chemoproteomics: Orbitrap-TMT vs timsTOF-diaPASEF
Chemoproteomics relies on precise and comprehensive quantification of protein–compound interactions, yet methodological comparisons across mass spectrometry platforms remain scarce. Here, we systematically benchmark tandem mass tag data-dependent acquisition (TMT-DDA) on an Orbitrap against label-fr...
ORGANISM(S): Homo sapiens (Human) 
2026-09-21 | PXD065032 | Pride
Dia Tmt Chemoproteomics Timstof Orbitrap Lc-msms 
ProteomicsEvaluation of deep and dynamic proteomic screening strategies at sub-50Hz scan rate and without automation
In this study, we systematically evaluate sample preparation strategies, data acquisition modes and data-analysis approaches for both labeled and label-free proteomics, with the explicit goal of maximizing throughput and quantitative depth using Orbitrap-based instruments operating below 50Hz scan r...
ORGANISM(S): Homo sapiens (Human) 
2026-03-27 | PXD073468 | Pride
UnknownIsobaric Tagging and Data Independent Acquisition as Complementary Strategies for Proteome Profiling on an Orbitrap Astral Mass Spectrometer.
Not available
S-EPMC11702835 | biostudies-literature
ProteomicsComparative proteome signatures of trace samples by multiplexed Data-Independent Acquisition
Single cell transcriptomics have revolutionized fundamental understanding of basic biology and disease. Since transcripts often do not correlate with protein expression, it is paramount to complement transcriptomics approaches with proteome analysis at single cell resolution. Despite continuous tech...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2021-05-17 | PXD023574 | Pride
Dia-tmt Identification-independent Ultra-low input samples 
ProteomicsEvaluation of deep and dynamic proteomic screening strategies at sub-50Hz scan rate and without automation
In this study, we systematically evaluate sample preparation strategies, data acquisition modes and data-analysis approaches for both labeled and label-free proteomics, with the explicit goal of maximizing throughput and quantitative depth using Orbitrap-based instruments operating below 50Hz scan ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Staphylococcus aureus 
2026-03-27 | PXD073367 | Pride
Sort   by:  
 Page size