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ProteomicsInfluence of digestion protocol in Protein Quantitation
Quantitative determination of absolute and relative protein amounts is an essential requirement for most current bottom-up proteomics applications, but protein quantitation estimates are affected by several sources of variability such as sample preparation, mass spectrometric acquisition, and data a...
ORGANISM(S): Escherichia coli 
2014-07-30 | PXD001187 | Pride
Protein quantitation Digestion protocols Fasp 
ProteomicsAssessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics
In this study, we evaluated three well-established sample preparation methods for shotgun proteomics, named: i) UREA; ii) sodium dodecyl sulfate (SDS); and iii) filter-aided sample preparation (FASP) protocols. We focused our analysis on protein extraction and digestion from heart left ventricle (LV...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-05-21 | PXD012591 | Pride
Lc-msms Rat Dimethyl labeling Heart Fasp 
UnknownQuantitative assessment of in-solution digestion efficiency identifies optimal protocols for unbiased protein analysis.
Not available
S-EPMC3790306 | biostudies-literature
ProteomicsQuantitative assessment of the effects of trypsin digestion methods on immunoprecipitation-mass spectrometry-based protein-protein interaction analysis
Immunoprecipitation-mass spectrometry (IP-MS) has become the method of choice for discovering protein-protein interaction (PPI) under native conditions. The success of the IP-MS depends on the efficiency of trypsin digestion and recovery of the tryptic peptides for MS analysis. Several different pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) 
2017-07-25 | PXD005984 | Pride
Rela interactome Trypsin digestion Protein-protein interaction 
UnknownExperimental Protocols Used to Mimic Gastrointestinal Protein Digestion: A Systematic Review.
Not available
S-EPMC11314321 | biostudies-literature
ProteomicsQuantitative assessment of the effects of trypsin digestion methods on immunoprecipitation-mass spectrometry-based protein-protein interaction analysis - part2
Immunoprecipitation-mass spectrometry (IP-MS) has become the method of choice for discovering protein-protein interaction (PPI) under native conditions. The success of the IP-MS depends on the efficiency of trypsin digestion and recovery of the tryptic peptides for MS analysis. Several different pro...
ORGANISM(S): Homo sapiens (Human) 
2017-07-26 | PXD006739 | Pride
Protein-protein interaction Trypsin digestion Lc-ms/ms 
UnknownPredicting the Glycemic Index of Biscuits Using Static In Vitro Digestion Protocols.
Not available
S-EPMC9858452 | biostudies-literature
UnknownCustomized digestion protocols for copepods, euphausiids, chaetognaths and fish larvae facilitate the isolation of ingested microplastics.
Not available
S-EPMC11358325 | biostudies-literature
ProteomicsComparison of in-gel digestion methods for histone PTM analysis
In order to optimize the histone digestion procedure for low-abundance clinical samples, we compared different in-gel digestion protocols. Histones are usually digested in-gel using “Arg-C-like” strategies, which involve chemical acylation of lysines followed by trypsin digestion (17). Acylated lysi...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD024745 | Pride
Epigenetics Histone post-translationa modification 
GenomicsAdult mouse lung digestion test
Adult mouse lung digestion test
PRJNA827142 | ENA
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