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ProteomicsEstablishment of dimethyl labeling-based quantitative acetylproteomics in Arabidopsis
Protein acetylation, one of many types of post-translational modifications (PTMs), is involved in a variety of biological and cellular processes. In the present study, we applied both CsCl density gradient (CDG) centrifugation-based protein fractionation and a dimethyl-labelling-based 4C quantitativ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-03-14 | PXD006177 | Pride
Cscl density gradient (cdg) centrifugation Stable isotope dimethyl labelling Arabidopsis Squa-d Acetylproteomics 4c proteomic workflow Quantitative ptm proteomics 
Metabolomics13C glucose labelling studies using 2D NMR is a useful tool for determining ex vivo whole organ metabolism during hypothermic machine perfusion of kidneys
Background: The aim of this study is to determine the feasibility of using Nuclear Magnetic Resonance (NMR) tracer studies (13C-enriched glucose) to detect ex vivo de novo metabolism in the perfusion fluid and cortical tissue of porcine kidneys during hypothermic machine perfusion (HMP). Methods: Po...
2017-10-16 | MTBLS367 | MetaboLights
ProteomicsComparative shotgun proteomics in wt-SUM44 and SUM44-LTED, using RIME and dimethyl-labelling
Wild type (wt) SUM44 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the ...
ORGANISM(S): Homo sapiens (Human) 
2017-12-11 | PXD004807 | Pride
Breast cancer Rime Dimethyl-labelling Sum44 
ProteomicsQuantitative Myogenesis C2C12 Proteome with Dimethyl-Labelling
The Z-disc is a protein-rich structure critically important for myofibril development and integrity. In order to monitor the quantiative changes in C2C12 myoblast during myogenesis, a quantitative dimethyl-labelling approach was performed with d0 myoblasts, d5 myotubes and electrical puls stimulated...
ORGANISM(S): Mus musculus (Mouse) 
2017-01-02 | PXD004960 | Pride
Muscle differentiation Myoblasts Dimethyl C2c12 cells Myotubes 
ProteomicsBSA Standard Dimethyl labelling 2
BSA digest standards - dimethyl labelling. Analysed by MALDI
ORGANISM(S): Bos Taurus (ncbitaxon:9913) 
2016-11-18 | MSV000080345 | MassIVE
ProteomicsE. coli spiking Dimethyl labelling
E coli spiked with BSA, Phos B, Myo, Trans - dimethyl labelling
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2016-11-18 | MSV000080346 | MassIVE
ProteomicsQuantitative proteomics on wt-MCF7 versus their LTED derivatives using dimethyl labelling
Wild type (wt) MCF7 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the a...
ORGANISM(S): Homo sapiens (Human) 
2016-06-01 | PXD004085 | Pride
Mcf7 Lted Dimethyl labelling 
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2021-09-09 | PXD022794 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
ProteomicsDimethyl labeling-based quantitative PTM proteomic analysis of drought-regulated phosphoproteomes in soybean drought-tolerant cultivar
The isotopic dimethyl labeling-based quantitative post-translational modification proteomics was applied to study the phosphoproteomic changes associated with the drought responses of two contrasting soybean cultivars. A total of 9,457 non-redundant, unambiguous, label-independent and repeatable pho...
ORGANISM(S): Glycine max 
2023-05-10 | PXD022806 | Pride
Dimethyl labelling Quantitative ptm proteomics Orbitrap fusion lumos Phosphoproteomics Glycine max Overly post-translationally modified protein (opp) Soybean drought-tolerant cultivar 
TranscriptomicsMicroRNA expression profiling of human differentiated HepaRG cells after exposure to rifampicin or efavirenz compared to exposure with dimethyl sulfoxide as control
Rifampicin and efavirenz are commonly used medicines to treat tuberculosis (rifampicin) and HIV/AIDS (efavirenz). Rifampicin and efavirenz are metabolized in the liver and exposure to these medicines can alter microRNA expression in hepatocytes. Changes in microRNA expression may affect metabolism o...
ORGANISM(S): Homo sapiens 
2021-12-31 | E-MTAB-7929 | biostudies-arrayexpress
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