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ProteomicsEstablishment of dimethyl labeling-based quantitative acetylproteomics in Arabidopsis
Protein acetylation, one of many types of post-translational modifications (PTMs), is involved in a variety of biological and cellular processes. In the present study, we applied both CsCl density gradient (CDG) centrifugation-based protein fractionation and a dimethyl-labelling-based 4C quantitativ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-03-14 | PXD006177 | Pride
Cscl density gradient (cdg) centrifugation Stable isotope dimethyl labelling Arabidopsis Squa-d Acetylproteomics 4c proteomic workflow Quantitative ptm proteomics 
ProteomicsComparative shotgun proteomics in wt-SUM44 and SUM44-LTED, using RIME and dimethyl-labelling
Wild type (wt) SUM44 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the ...
ORGANISM(S): Homo sapiens (Human) 
2017-12-11 | PXD004807 | Pride
Breast cancer Rime Dimethyl-labelling Sum44 
ProteomicsBSA Standard Dimethyl labelling 2
BSA digest standards - dimethyl labelling. Analysed by MALDI
ORGANISM(S): Bos Taurus (ncbitaxon:9913) 
2016-11-18 | MSV000080345 | MassIVE
ProteomicsE. coli spiking Dimethyl labelling
E coli spiked with BSA, Phos B, Myo, Trans - dimethyl labelling
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2016-11-18 | MSV000080346 | MassIVE
ProteomicsQuantitative Myogenesis C2C12 Proteome with Dimethyl-Labelling
The Z-disc is a protein-rich structure critically important for myofibril development and integrity. In order to monitor the quantiative changes in C2C12 myoblast during myogenesis, a quantitative dimethyl-labelling approach was performed with d0 myoblasts, d5 myotubes and electrical puls stimulated...
ORGANISM(S): Mus musculus (Mouse) 
2017-01-02 | PXD004960 | Pride
Muscle differentiation Myoblasts Dimethyl C2c12 cells Myotubes 
ProteomicsQuantitative proteomics on wt-MCF7 versus their LTED derivatives using dimethyl labelling
Wild type (wt) MCF7 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the a...
ORGANISM(S): Homo sapiens (Human) 
2016-06-01 | PXD004085 | Pride
Mcf7 Lted Dimethyl labelling 
ProteomicsDimethyl-Labelling of wild-type yeast treated with hydrogen peroxide LC-MS/MS
Changes of protein abundance upon H2O2 treatment of wild-type yeast cells
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-01-24 | PXD003584 | Pride
Dimethyl-labelling Yeast Oxidative stress Lc-ms/ms 
ProteomicsQuantitative proteomics analysis of the Arg/N-end rule pathway of targeted degradation in Arabidopsis roots
According to the Arg/N-end rule pathway, proteins with basic N-termini are targeted for degradation by the Arabidopsis thaliana E3 ligase, PROTEOLYSIS6 (PRT6). Proteins can also become PRT6 substrates following post-translational arginylation by arginyltransferases ATE1 and 2. Here, we undertook a q...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-03-26 | PXD001719 | Pride
Tmt N-end rule Root Tails Quantitative proteomics 
ProteomicsPhosphoproteomics of mouse cardiomyocytes reveals signalling pathways triggered by a vasodilator cryptide
Animal venoms are natural sources of bioactive molecules such as cryptides that consist in biological active peptides released after proteolytic cleavage of a peptide or protein precursor. The KPP is a cryptided derived from the c-terminal of an hypotensin (TsHpI) a peptide isolated from the venom o...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-06 | PXD015068 | Pride
Hipotensins Venome Scorpion Cryptide Cardiomyocytes Phosphopreteomics 
ProteomicsBSA Standard Dimethyl labelling
BSA digest standards, MD and PS, maXis and ultraflex
ORGANISM(S): Bos Taurus (ncbitaxon:9913) 
2016-11-18 | MSV000080344 | MassIVE
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