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ProteomicsMorpheus Spectral Counter - Morpheus spectral counter: a computational tool for label-free quantitative mass spectrometry using the morpheus search engine
Label-free quantitative mass spectrometry (MS) based on the Normalized Spectral Abundance Factor (NSAF) has emerged as a simple and reasonably robust method to determine the relative abundance of individual proteins within complex mixtures. Here, we describe Morpheus Spectral Counter (MSpC) as the ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-02-24 | PXD003002 | Pride
Proteomics Morpheus Software Bioinformatics Spectral count Label-free quantification Lc-ms/ms 
UnknownMorpheus Spectral Counter: A computational tool for label-free quantitative mass spectrometry using the Morpheus search engine.
Not available
S-EPMC5089804 | biostudies-literature
UnknownEvaluating Chromatographic Approaches for the Quantitative Analysis of a Human Proteome on Orbitrap-Based Mass Spectrometry Systems.
Not available
S-EPMC6839767 | biostudies-literature
ProteomicsComammox - Complete nitrification by Nitrospira bacteria
Nitrification, the oxidation of ammonia via nitrite to nitrate, has always been considered to be a two-step process catalysed by chemolithoautotrophic microorganisms oxidizing either ammonia or nitrite. No known nitrifier carries out both steps, although complete nitrification should be energeticall...
ORGANISM(S): Candidatus Nitrospira inopinata 
2019-03-15 | PXD013103 | Pride
Nitrogen Lc-ms Culture Nitrification Microbes 
ProteomicsOptimizing chromatographic conditions for orbitrap-based MS platforms
We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa...
ORGANISM(S): Homo sapiens (Human) 
2019-03-04 | PXD009875 | Pride
Reproducibility Data-dependent acquisition Chromatography Q exactive Fusion lumos Human Pearson product-moment correlation coefficient Distributed normalized spectral abundance factor Orbitrap Quantitative proteomics Multidimensional protein identification technology 
MetabolomicsResolving coral photoacclimation dynamics through coupled photophysiological and metabolomic profiling
Corals continuously adjust to short term variation in light availability on shallow reefs. Long-term light alterations can also occur due to natural and anthropogenic stressors, as well as management interventions such as coral transplantation. Although short term photophysiological responses are re...
2019-04-11 | MTBLS768 | MetaboLights
ProteomicsProteomic changes induced by cold challenge in gilthead sea bream (Sparus aurata) liver
We report the proteomic characterization of livers from Sparus aurata exposed to cold temperatures. In this study, mimicking the winter challenge conditions, a 8 week feeding trial was carried out on gilthead sea bream juveniles reared in RAS systems at a temperature ramp made of two phases of four...
ORGANISM(S): Sparus aurata 
2019-05-28 | PXD011059 | Pride
Proteomics Sparus aurata Liver Cold exposure Lc-ms/ms 
ProteomicsProteome of silenced minichromosome maintenance protein 2 with siRNA on non-small cell lung cancer H1299 cells
Lung cancer is the leading cause of cancer-related mortality. The two main lung cancer types are small cell lung cancer (SCLC) and non-SCLC (NSCLC), where NSCLC comprises about 80-85% of all lung cancer. Despite the introduction of improved treatments, the overall survival rate of lung cancer patien...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD003743 | Pride
Non small cell lung cancer h1299 cells Rna interference Proteome Minichromosome maintenance protein 2 Itraq Lc-ms/ms 
ProteomicsMolecular evolution of juvenile hormone esterase-like proteins in a socially exchanged fluid
Socially exchanged fluids, such as seminal fluid and milk, are a direct means through which an organism can influence conspecifics. When orally feeding larval offspring via trophallaxis, workers of the carpenter ant Camponotus floridanus were recently shown to transfer Juvenile Hormone III (JH), a k...
ORGANISM(S): Camponotus floridanus 
2018-12-14 | PXD009982 | Pride
Ants Larva Camponotus floridanus 
ProteomicsProteomic changes occurring along gonad maturation in the edible sea urchin Paracentrotus lividus
We report the proteomic characterization of gonads from wild P. lividus collected along coastal Sardinia, and describe the changes occurring in gonads according to sex and developmental stage. Gonads in the recovery, pre-mature, mature, and spent stages were analyzed using a shotgun proteomics appro...
ORGANISM(S): Paracentrotus lividus (Common sea urchin) 
2016-06-09 | PXD004200 | Pride
Proteomics Paracentrotus lividus gonads Lc-ms/ms Gonads maturation 
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