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ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of digestion solution. In this study we characterized ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on the ...
ORGANISM(S): Homo sapiens (Human) Loxodonta africana (African elephant) 
2020-10-19 | PXD019039 | Pride
Sequence coverage Proline-rich Disulfide mapping Low-ph Proteomics Phosphorylation De novo sequencing Disulfide bonds Proalanase 
ProteomicsA Novel Mechanism Mediated by Allosteric Disulfide Bonds Fine-Tunes Thrombus Formation
Mapping of disulfide bonds and quantification of their redox state in the human histidine rich glycoprotein
ORGANISM(S): Homo sapiens (Human) 
2024-04-11 | PXD050718 | Pride
Histidine rich glycoprotein Hrg Disulfide bonds Redox state 
ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping_part2
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of digestion solution. In this study we characterized ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on the ...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021191 | Pride
Sequence coverage Proline-rich Disulfide mapping Low-ph Proteomics Phosphorylation De novo sequencing Disulfide bonds Histone ptms Proalanase Histone variants 
ProteomicsProAlanase is an effective alternative to trypsin for proteomics applications and disulfide bond mapping_part3
Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of the digestion solution. In this study we characterize ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on t...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD021703 | Pride
Sequence coverage Proline-rich Low-ph Histones Proteomics Phosphorylation De novo sequencing Disulfide bonds Proalanase 
ProteomicsPfs25 Disulfide Mapping
Disulfide bond mapping of recombinant Pfs25 produced in Baculovirus. Mass Spectrometry Data set
ORGANISM(S): Plasmodium Falciparum (ncbitaxon:5833) 
2018-01-28 | MSV000081982 | MassIVE
ProteomicsA2M Thr654Cys Thr661Cys mutant disulfide mapping
The Thr654Cys and Thr661Cys mutations were used to introduce a disulfide bridge into the interacting LNK domains of human A2M. In this study, formation of the disulfide was investigated by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2021-05-21 | PXD019081 | Pride
Human a2m disulfide mutant 
ProteomicsFast and Accurate Disulfide Bridge Detection
Recombinant expression of proteins, propelled by therapeutic antibodies, has evolved into a multi-billion-dollar industry. Essential here is quality control assessment of critical attributes such as sequence fidelity, proper folding, and post-translational modifications (PTMs). Errors can lead to di...
ORGANISM(S): Homo sapiens (Human) 
2024-04-08 | PXD046855 | Pride
Faims Disulfide Ethcd Mab Scrambling 
UnknownAcetone/Isopropanol Photoinitiating System Enables Tunable Disulfide Reduction and Disulfide Mapping via Tandem Mass Spectrometry.
Not available
S-EPMC6310128 | biostudies-literature
ProteomicsDisulfide mapping of J-chain by crosslinking MS
Here we show how J-chain (JC) outcompetes the sixth IgM subunit during assembly. We looked at the disulfide re-arrangements in JC and showed that it exists as an unstructured protein with non-native disulfides before its insertion into IgM.
ORGANISM(S): Mus musculus (Mouse) 
2024-12-04 | PXD048614 | Pride
Disulfide J-chain Crosslinking 
UnknownMass Spectrometry-Based Disulfide Mapping of Lysyl Oxidase-like 2.
Not available
S-EPMC9180022 | biostudies-literature
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