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ProteomicsProtein idetification by using DNA pull-down & MS assay
Identification of proteins binding to the promoter of hrpR gene in Pseudomonas syringae using DNA pull-down & MS assay
ORGANISM(S): Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 
2021-03-23 | PXD023722 | Pride
Promoter Dna pull-down Lc-ms/ms 
ProteomicsDNA pulldown of PRDM16 promoter and measurement of LC/MS-MS
To further investigate the transcription cofactor of Smad3 that regulated the transcription of PRDM16, we conducted DNA pull-down assay. we treated HK-2 cells with TGF-β and extracted the nuclear proteins. And the nuclear proteins were incubated with streptavidin magnetic beads, and biotin-labeled D...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD044753 | Pride
Hk-2 cell line dna pull-down lc/ms-ms 
GenomicsDeep sequencing of HetR-bound DNA reveals novel HetR targets in Anabaena sp. strain PCC 7120
We employed chromatin pull-down and deep sequencing to globally identify HetR DNA targets in vivo at 6 hours after fixed-nitrogen deprivation. We identified novel DNA binding targets of tagged HetR-6xHis and defined a consensus HetR binding site from these HetR target sequences. Chromatin pull down ...
ORGANISM(S): Nostoc sp. PCC 7120 
2013-10-31 | E-GEOD-51886 | biostudies-arrayexpress
ProteomicsDNA pull-down and mass spectrometry
DNA pull-down and mass spectrometry analysis were performed by utilizing a biotin-labeled human PDPN promoter.
ORGANISM(S): Homo sapiens (Human) 
2026-03-13 | PXD067543 | Pride
Human nasal epithelial cells Coomassie brilliant blue staining Lc-ms Dna pulldown 
ProteomicsA quantitative proteomics tool to identify DNA-protein interactions in primary cells or blood
The interaction between transcription factors and genomic DNA forms the basis for spatio-temporal control of gene expression. Therefore, these interactions and their impact on disease and cellular fate are extensively studied on a global level, mainly using techniques based on next-generation sequen...
ORGANISM(S): Homo sapiens (Human) 
2015-01-05 | PXD000782 | Pride
Blood Pbmcs Dna pull-down Primary cells 
UnknownVersatile and efficient chromatin pull-down methodology based on DNA triple helix formation.
Not available
S-EPMC5897567 | biostudies-literature
ProteomicsDNA pull down between WT and nos
DNA pull down was performed to identify icaA promoter-bound proteins
ORGANISM(S): Staphylococcus Aureus 
2024-10-30 | PXD057339 |
GenomicsProteomic and Genomic Analysis of transcription factor TFII-I reveals insight into the response to cellular stress
We expressed biotin-tagged transcription factor TFII-I (GTF2I) in K562 cells and subjected crosslinked chromatin to streptavidin mediated pull down. We subjected the isolated DNA to Illumina sequencing. We identified 18920 TFII-I binding peaks. Nearest gene analysis revealed 5886 genes associated w...
ORGANISM(S): Homo sapiens 
2014-05-14 | E-GEOD-51065 | biostudies-arrayexpress
TranscriptomicsTcRBP40 pull-down ribonomics
RNA pull-down assay.
For the recombinant protein pull-down assays, 50 M-5g of recombinant His-tag TcRBP40 protein were bound to 100 uL of Ni-NTA resin (Qiagen) overnight at 4M-0C. 100 M-5g of total RNA from epimastigotes were incubated with the bound protein in 500 M-5l EMSA buffer at 4M-0C for 2...
ORGANISM(S): Trypanosoma cruzi 
2012-06-30 | E-MEXP-3057 | biostudies-arrayexpress
Methylation profilingSequencing analysis for 5hmC pull-down DNA
The gene body regions of cardiomyocyte-specific genes in cardiomyocytes are hypomethylated. We confirmed that the DNA methylation in gene body regions were demethylated during development. We speculated that the demethylation of gene body regions in cardiomyocyte-specific genes might be associated w...
ORGANISM(S): Mus musculus 
2016-09-22 | GSE87165 | GEO
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