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GenomicsPost-licensing specification of eukaryotic replication origins by facilitated Mcm2-7 sliding along DNA
Eukaryotic genomes are replicated from many origin sites that are licensed by the loading of inactive double hexamers of the replicative DNA helicase, Mcm2-7. How eukaryotic origin positions are specified remains elusive. Here we show that, contrary to the bacterial paradigm, eukaryotic origins are ...
ORGANISM(S): Saccharomyces cerevisiae 
2016-01-07 | E-GEOD-69065 | biostudies-arrayexpress
ProteomicsCRL2LRR1 is required for CMG unloading during vertebrate replication termination
A key event during eukaryotic replication termination is the removal of the CMG helicase from chromatin. CMG unloading involves ubiquitylation of its MCM7 subunit and the action of the p97 ATPase. Using a proteomic screen in Xenopus egg extracts, we identified factors that are retained on chromatin...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2017-02-02 | PXD004828 | Pride
Chromass Dna replication termination Cullin e3 ligases Xenopus egg extracts 
ProteomicsReplisome composition of stalled, terminating replication forks
Topological stress can cause replication forks to stall as they converge upon one another during termination of vertebrate DNA synthesis. However, replication forks ultimately overcome topological stress and complete DNA synthesis, suggesting that alternative mechanisms can overcome topological stre...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2023-02-21 | PXD031170 | Pride
Xenopus Lc-msms Topoisomerase Egg extracts Termination Dna replication 
GenomicsReplication termination in cells lacking 3' exonucleases and RecG helicase
Termination of bacteril DNA replication in the absence of processing enzymes
PRJEB85678 | ENA
GenomicsNELF coordinates Pol II transcription termination and DNA replication
NELF coordinates Pol II transcription termination and DNA replication
PRJNA1250570 | ENA
TranscriptomicsQuantitative, genome-wide analysis of eukaryotic replication initiation and termination
Via the deep-sequencing of Okazaki fragments from Saccharomyces cerevisiae, we report the first comprehensive documentation of genome-wide replication directionality in any eukaryote; this permits the systematic analysis of both replication initiation and termination. We conduct a genome-wide analys...
ORGANISM(S): Saccharomyces cerevisiae 
2013-02-28 | E-GEOD-40696 | biostudies-arrayexpress
TranscriptomicsHigh-resolution genome replication profiles, modeling and single-cell imaging define the stochastic nature of replication initiation and termination
Eukaryotic genome replication is stochastic with each cell using a different cohort of replication origins. Interpreting high-resolution genome replication profiles with a mathematical model allowed us to quantify the stochastic nature of genome replication. This approach included estimation of the ...
ORGANISM(S): Saccharomyces cerevisiae 
2013-11-07 | E-GEOD-48561 | biostudies-arrayexpress
TranscriptomicsA global profile of replication polymerase usage
We have established a novel sequencing approach to characterise usage of replicative DNA polymerases in S. pombe. This approach allows us to determine the roles of DNA polymerase delta and epsilon in lagging strand and leading strand DNA synthesis, respectively in genome-wide scale. Utilising the d...
ORGANISM(S): Schizosaccharomyces pombe 
2015-02-03 | E-GEOD-62108 | biostudies-arrayexpress
Genomics3' exonucleases in termination
Role of 3' and 5' exonucleases in the termination of DNA replication
PRJEB28600 | ENA
UnknownTermination of DNA replication drives genomic instability via multiple mechanisms.
Not available
S-EPMC12809603 | biostudies-literature
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