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ProteomicsProteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links, part 1 of 2: CHROMASS DATA
DNA interstrand crosslinks (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. Here we establi...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2015-05-07 | PXD000490 | Pride
Icl repair Chromatin Stalled replication forks Dna damage response 
ProteomicsProtein recruitment on chromatin in the presence or absence of Plk1
Polo-like kinase 1 (Plk1) is an important protein kinase for checkpoint recovery and adaptation in response to DNA damage and replication stress. However, although Plk1 is present in S phase, little is known about its localization and function during unperturbed DNA replication. Here we used a previ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2021-08-25 | PXD022572 | Pride
Xenopus laevis Chromass Lcmsms 
ProteomicsSCAI orchestrates DNA interstrand crosslink repair downstream of FANCI-FANCD2 ubiquitylation
By covalently linking Watson and Crick strands, DNA interstrand crosslinks (ICLs) are highly toxic lesions that block DNA replication and threaten genome integrity. The Fanconi anemia (FA) pathway orchestrates ICL repair during DNA replication, with ubiquitylated FANCI-FANCD2 (ID2) marking the activ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2022-02-18 | PXD024280 | Pride
Scai Xenopus Interstrand crosslink repair Chromass Dna damage Icl Fanci Fancd2 
ProteomicsProteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links part 2 of 2: AP-MS DATA (QUBIC)
DNA interstrand crosslinks (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. Here we establi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-13 | MSV000079577 | MassIVE
UnknownDNA repair. Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links.
Not available
S-EPMC5331883 | biostudies-literature
ProteomicsActivation of the ATR kinase by the RPA-binding protein ETAA1
The ATR kinase is a master regulator of cellular responses to DNA damage and replication stress that is activated by a complex mechanism involving its binding partner ATRIP, RPA-coated ssDNA, the 9-1-1 complex and TopBP1. Here, we discovered a new ATR activation mechanism in human cells, mediated by...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2016-09-30 | PXD003529 | Pride
Dsb repair Chromass Dna damage response 
ProteomicsBipartite GINS binding mode of TopBP1 to activate the replicative helicase - Chromass Data Set
How the replicative Mcm2-7 helicase is activated during replication origin firing remains largely unknown. Our biochemical and structural studies reveal that the helicase activator GINS interacts with TopBP1 using two separate binding surfaces. One involves a stretch of highly conserved amino acids ...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-03-26 | PXD040024 | Pride
Xenopus egg extracts Dna replication 
ProteomicsProteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links part 2 of 2: AP-MS DATA (QUBIC)
DNA interstrand crosslinks (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. Here we establi...
ORGANISM(S): Homo sapiens (Human) 
2015-05-07 | PXD000491 | Pride
Icl repair Chromatin Stalled replication forks Dna damage response 
ProteomicsCRL2LRR1 is required for CMG unloading during vertebrate replication termination
A key event during eukaryotic replication termination is the removal of the CMG helicase from chromatin. CMG unloading involves ubiquitylation of its MCM7 subunit and the action of the p97 ATPase. Using a proteomic screen in Xenopus egg extracts, we identified factors that are retained on chromatin...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2017-02-02 | PXD004828 | Pride
Chromass Dna replication termination Cullin e3 ligases Xenopus egg extracts 
ProteomicsMechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO pathway
DNA-protein crosslinks (DPCs) obstruct essential DNA transactions, posing a serious threat to genome stability and functionality. DPCs are proteolytically processed in a ubiquitin- and DNA replication-dependent manner by SPRTN or the proteasome but can also be resolved via targeted SUMOylation. Howe...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2021-08-06 | PXD021947 | Pride
Rnf4 Xenopus Dpc Chromass Dna damage Replication Sumo Pias4 
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