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ProteomicsSFB-tagged(S-protein tag, Flag epitope tag, and streptavidin-binding peptide tag) RNF4 wild-type and CS mutant LC-MSMS
To elucidate the role of RNF4 in DNA replication and fork reversal, we performed tandem affinity purification (TAP) using a HEK293T cell line that stably expresses SFB-tagged(S-protein tag, Flag epitope tag, and streptavidin-binding peptide tag) wild-type and CS mutant to isolate proteins that assoc...
ORGANISM(S): Homo sapiens (Human) 
2022-08-11 | PXD028397 | Pride
Human rnf4 wt and cs lc-msms 
ProteomicsRNF4 interactome in heatshocked HeLa cells
Exposure of cells to heat or oxidative stress causes misfolding of proteins. To avoid toxic protein aggregation, cells have evolved nuclear and cytosolic protein quality control (PQC) systems. In response to proteotoxic stress cells also limit protein synthesis by triggering transient storage of mRN...
ORGANISM(S): Homo sapiens (Human) 
2023-06-30 | PXD017888 | Pride
Hela rnf4 heatshock interactome 
ProteomicsRNF4-knockdown and RNF4-TULIP identified proteins
In this project we aim to identify the spacific targets of the SUMO-Targeted Ubiquitin Ligase (STUBL) RNF4. For that, we combine the study of SUMOylation targets that are enriched after RNF4 knockdown with the use of Targeted Ubiquitin Ligases Identified by Proteomics (TULIP).
ORGANISM(S): Homo sapiens (Human) 
2017-11-23 | PXD005425 | Pride
Sumo Rnf4 Ubiquitin 
ProteomicsWhole cell proteome of HeLa Flp in T-RexTM RNF4 WT treated with either CCW16, 1a or 2c
The SUMO-targeted E3 ubiquitin ligase RNF4 plays an important role in safeguarding genome and proteome integrity. RNF4 recognizes polySUMO modified proteins and induces their proteolytic or non-proteolytic ubiquitylation. Based on the key functions of RNF4 maintaining proteome and genome stability, ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD056951 | Pride
Rnf4 Tmt Wcp Ccw16 Mammalian cells 
GenomicsIdentification of RNF4 dependent gene signature in melanoma tumors
Identification of RNF4 dependent gene signature in melanoma tumors
PRJNA510643 | ENA
ProteomicsIdentification of heat induced, RNF4 regulated ubiquitylation sites
Exposure of cells to heat or oxidative stress causes misfolding of proteins. To avoid toxic protein aggregation, cells have evolved nuclear and cytosolic protein quality control (PQC) systems. In response to proteotoxic stress cells also limit protein synthesis by triggering transient storage of mRN...
ORGANISM(S): Homo sapiens (Human) 
2023-06-30 | PXD017887 | Pride
Ubiquitylation heatshock rnf4 hela 
GenomicsRNF4-RGMb axis is required for osteogenic differentiation and cancer cell survival
RNF4-RGMb axis is required for osteogenic differentiation and cancer cell survival
PRJNA845209 | ENA
TranscriptomicsIdentification of RNF4 dependent gene signature in melanoma tumors
Among hallmarks of cancer is impaired proteostasis, that is characterized by increased oncoprotein stability. Here we demonstrate that RNF4, a ubiquitin ligase implicated in the stabilization of short-lived oncoproteins, is important for melanoma tumorigenesis. High levels of RNF4 in melanoma patien...
ORGANISM(S): Homo sapiens 
2021-05-05 | GSE124064 | GEO
GenomicsEffect of RNF4 depletion on GSK3685032-induced gene expression changes in MV4-11 cells [RRBS]
Effect of RNF4 depletion on GSK3685032-induced gene expression changes in MV4-11 cells [RRBS]
PRJNA977661 | ENA
TranscriptomicsRNF4-RGMb axis is required for osteogenic differentiation and cancer cell survival
Molecular understanding of osteogenic differentiation (OD) of human bone-marrow derived mesenchymal stem cells (hBMSCs) is important for regenerative medicine and has direct implications to cancer. Here we report that the RNF4 ubiquitin ligase is essential for OD of hBMSCs, and that RNF4 deficient h...
ORGANISM(S): Homo sapiens 
2022-09-28 | GSE205432 | GEO
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