We applied TMT-AP-MS to study protein clients between wild type and different mutations of DNAJB1 and DNJAB8 in HEK293T cells. We also applied heat to see how the interactome changes between different types of bait.
We applied TMT-AP-MS to determine changes in Flag-DNAJB8-H31Q interactors in HEK293T cells upon treatment with acetochlor, alachlor, and propachlor. We also looked into aggregation in HEK293T cells after propachlor treatment.