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ProteomicsE2-Ub photocrosslinking probes in HeLa cells
Label-free quantitative (LFQ) mass spectrometry to determine the interactome of E2-Ub probe in native HeLa cells;
ORGANISM(S): Homo sapiens (Human) 
2026-02-06 | PXD059494 | Pride
E2-ub probe 
ProteomicsE2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells
The E2 ubiquitin (Ub)-conjugating enzyme primarily determines Ub conjugation as Ub-isopeptide (lysine), Ub-oxyester (serine/threonine) or Ub-thioester (cysteine). However, E2-specific Ub conjugation profiles within cells remain elusive. Here, we developed the fusion E2–Ub-R74G profiling (FUSEP) stra...
ORGANISM(S): Homo Sapiens 
2024-12-11 | PXD058017 |
ProteomicsZNFX1 activity-based probe crosslinking mass spectrometry
The aim of this experiment was to identify the catalytic cysteine in the antiviral E3 ligase ZNFX1 using activity-based probe (ABP)–enabled cross-linking mass spectrometry (XL-MS). A biotinylated E2~ubiquitin activity-based probe containing a cysteine-electrophile trap was used to covalently capture...
ORGANISM(S): Homo sapiens (Human) 
2026-05-22 | PXD073419 | Pride
ProteomicsSmall-Molecule Activity-Based Probe for Monitoring Ubiquitin 2 C‑Terminal Hydrolase (UCHL1) Activity in Live Cells and Zebrafish 3 Embryos
Many reagents have emerged to study the function of specific enzymes in vitro. On the other hand, target specific reagents are scarce or need improvement, allowing investigations of the function of individual enzymes in their native cellular context. Here we report the development of a target-select...
ORGANISM(S): Homo sapiens (Human) 
2020-09-18 | PXD021557 | Pride
Orbitrap Human 
ProteomicsActivity-Based Ubiquitin-α-Amidomethyl Acrylate Probe for Profiling of Ubiquitinating Enzymes and Deubiquitinases
Activity-based ubiquitin probes have emerged as powerful tools for dissecting protein ubiquitination. In this work, we discovered that an α-amidomethyl substituted acrylate electrophile exhibits markedly enhanced reactivity. Based on this finding, we designed and synthesized a new activity-based pro...
ORGANISM(S): Homo Sapiens 
2026-06-30 | PXD080401 |
UnknownA cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes.
Not available
S-EPMC5108872 | biostudies-literature
ProteomicsPhotocrosslinking Activity-Based Probes to Capture the Dynamics of Ubiquitin RING E3 Ligase Interactions
Almost all cellular processes are influenced by ubiquitination. A large family of enzymes known as E3 ligases provide the specificity for ubiquitination, with the largest class among them, the RING E3s, comprising over 600 members in humans. RING E3s facilitate transfer of ubiquitin to substrates by...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075613 | Pride
Ubiquitin n-maleimido-diazirine e3 ligase activity-based probe 
UnknownUbFluor: A Mechanism-Based Probe for HECT E3 Ligases.
Not available
S-EPMC4965700 | biostudies-literature
ProteomicsIdentification by activity-based probes of a new Plasmodium falciparum E2 ubiquitin conjugating enzyme
The ubiquitin-proteasome system (UPS) is essential for Plasmodium falciparum survival and represents a potential target for antimalarial therapies. We utilized a ubiquitin-based activity probe (Ub-Dha) to capture active ubiquitin conjugating machinery during asexual blood-stage development. Several ...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2025-03-13 | PXD056473 | Pride
Plasmodium falciparum Activity proteomics Ubiquitin 
ProteomicsE3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP
RNF213 is a giant E3 ubiquitin ligase and a major susceptibility factor of Moyamoya disease, a cerebrovascular disorder that can result in stroke or death. In the cell, RNF213 is involved in lipid droplet formation, lipotoxicity, hypoxia, and NF-κB signaling, but its exact function in these processe...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2026-08-18 | PXD025992 | Pride
Rnf213 ube2l3 abp 
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