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ProteomicsN-terminal modification of eGFP by LC-MSMS
We used an in-depth analysis of the characteristics of eGFP as a proof-of-principle analyte to establish a robust assay for the determination of degradation rates on the single-cell level using microinjection and live-cell microscopy. We aimed to determine the influence of the exposed n-terminal ami...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-05-06 | PXD042569 | Pride
Lc-msms N-degron Ptm Egfp Acetylation 
TranscriptomicsComparison of Sox9-EGFP cell type transcriptomes during irradiation-induced crypt regeneration
Transcriptional profiling of intestinal epithelial cells expressing either Negative, Sublow, Low or High levels of the Sox9-EGFP reporter transgene FACS-isolated from jejunum of non-irradiated mice or at 5 days after 14Gy abdominal irradiation. 4 distinct cell populations, FACS-isolated based on exp...
ORGANISM(S): Mus musculus 
2012-06-17 | E-GEOD-32227 | biostudies-arrayexpress
TranscriptomicsCharacterization of IGF1 effects on the transcriptome of normal and irradiated Sox9-EGFP cell populations
Transcriptional profiling of intestinal epithelial cells expressing either Negative, Sublow, Low or High levels of the Sox9-EGFP reporter transgene FACS-isolated from jejunum of non-irradiated mice or at 5 days after 14Gy abdominal irradiation. In both groups, mice were treated for 5 consecutive day...
ORGANISM(S): Mus musculus 
2015-04-14 | E-GEOD-42817 | biostudies-arrayexpress
ProteomicsUbiquitination site analysis of eGFP by DARPin-based bioPROTACs analysed by LC-MS-MS
We investigated design principles of DARPin-based bioPROTACs targeting the model substrate GS-eGFP (eGFP with an n-terminal tag, necessary for proteasomal unfolding). Using a highly active E3 ligase domain from CHIP and nine well-characterized DARPins, we evaluated their ability to induce proteasoma...
ORGANISM(S): Homo sapiens (Human) 
2025-06-05 | PXD063877 | Pride
Ubiquitination Darpin Bioprotac Egfp 
TranscriptomicsAnalysis of pvalb-egfp cells from mouse visual cortex.
We provide an annotated cDNA clone collection which is particularly suitable for transcriptomic analysis in the mouse brain. Using it on microarrays, we compared the transcriptome of EGFP positive and negative cells in a parvalbumin-egfp transgenic background and showed that more than 30 % of clones...
ORGANISM(S): Mus musculus 
2008-08-22 | E-GEOD-8968 | biostudies-arrayexpress
GenomicsHomo sapiens strain:U2OS-EGFP
Homo sapiens strain:U2OS-EGFP Targeted Locus (Loci)
PRJNA233643 | ENA
MetabolomicsMetabolomic analysis of riboswitch containing E. coli recombinant expression system
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells. This metabolic burden analysis was performed against a number of recombinant expression systems and co...
2017-11-16 | MTBLS393 | MetaboLights
GenomicsATAC seq from EGFP+ and EGFP- cells from postnatal day 3 striatum from GENSAT Nr4a1-EGFP mice
ATAC seq from EGFP+ and EGFP- cells from postnatal day 3 striatum from GENSAT Nr4a1-EGFP mice
PRJNA601456 | ENA
GenomicsTranscriptome analysis of Mapt-EGFP+ and Mapt-EGFP- myenteric cells
Transcriptome analysis of Mapt-EGFP+ and Mapt-EGFP- myenteric cells
PRJNA822404 | ENA
TranscriptomicsExpression data from E10.5 mouse otocyst
We established a novel EGFP reporter mouse line (named Tg(ETAR-EGFP)14Imeg), which enables the placode-derived inner ear sensory cell lineage to be visualized and monitored. At E10.5, EGFP expression was detected in the ventral and dorsomedial region of the otocyst. Using this reporter line and FACS...
ORGANISM(S): Mus musculus 
2011-05-23 | E-GEOD-19618 | biostudies-arrayexpress
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